二斑叶螨为害对草莓叶片H_(2)O_(2)、MDA含量以及部分防御酶活性的影响  

Effects of Tetranychus urticae feeding on the H_(2)O_(2),MDA content and some defensive enzyme activities in strawberry leaves

作　　者：陈鹏 刘奇志 CHEN Peng;LIU Qi-Zhi(Shandong Provincial Engineering Technology Research Center on Biocontrol of Crops Diseases and Insect Pest,Institute of Plant Protection,Shandong Academy of Agricultural Sciences,Jinan 250100,China;College of Plant Protection,China Agricultural University,Beijing 100193,China)

机构地区：[1]山东省农业科学院植物保护研究所,山东省病虫害生物防治工程技术研究中心,济南250100 [2]中国农业大学植物保护学院,北京100193

出　　处：《环境昆虫学报》2022年第3期697-703,共7页Journal of Environmental Entomology

基　　金：国家科技支撑计划项目(2014BAD16B0702);山东省农业科学院农业科技创新工程(CXGC2021B13,CXGC2022E04)。

摘　　要：本文旨在探究二斑叶螨Tetranychus urticae为害对草莓Fragaria×ananassa Duch.叶片内过氧化氢(H_(2)O_(2))、丙二醛(MDA)含量以及部分防御酶活性的影响。在草莓苗上接种不同数量(5~25头)的二斑叶螨,分别在接种后的24 h、48 h和72 h取样,分析草莓叶片内H_(2)O_(2)、MDA的含量以及部分防御酶的活性。结果显示,二斑叶螨为害的草莓叶片内H_(2)O_(2)和MDA的含量以及超氧化物歧化酶(SOD)的活性随着时间的延长而呈现先升后降的趋势,在二斑叶螨持续为害草莓叶片24 h、48 h和72 h时,受损草莓叶片中H_(2)O_(2)的含量均显著高于对照(P<0.05),不同密度二斑叶螨为害的草莓叶片中H_(2)O_(2)的含量均显著高于对照(P<0.05),但与取食时间关系不大。当为害时间达到48 h时,MDA的含量和SOD的活性均达到最高峰,此时它们均与二斑叶螨的密度密切相关。当二斑叶螨为25头/叶时,MDA的含量和SOD的活性分别约是对照的3.6倍和10倍。过氧化物酶(POD)和过氧化氢酶(CAT)的活性随时间延长不断升高,均在二斑叶螨为害72 h时达到最高峰。同时,二斑叶螨的为害时间和为害密度之间存在一定的交互作用。以上结果表明草莓叶片主要通过调节其体内H_(2)O_(2)和MDA的含量以及各种防御酶活性的变化,对二斑叶螨的为害产生应激反应。This paper aimd to explore the effects of Tetranychus urticae damage on the content of hydrogen peroxide(H_(2)O_(2)),malondialdehyde(MDA)and some defensive enzyme activities in strawberry leaves.Inoculate strawberry seedlings with different numbers(5~25)of T.urticae,and sample at 24 h,48 h and 72 h after inoculation.The content of H_(2)O_(2),MDA and the changes of some defensive enzyme activities in strawberry leaves were analyzed.The results showed that the content of H_(2)O_(2) and MDA in the leaves of strawberries damaged by T.urticae and the activity of superoxide dismutase(SOD)showed a trend of first increasing and then decreasing with time.When T.urticae continued to damage strawberry leaves for 24 h,48 h and 72 h,H_(2)O_(2) content in the damaged strawberry leaves was significantly higher than control(P<0.05).The content of H_(2)O_(2) in strawberry leaves damaged by different densities of T.urticae was significantly higher than control(P<0.05),but it had little relationship with feeding time.When the damage time reached 48 h,MDA content and SOD activity both reached the highest peaks.At this time,they were closely related to the density of T.urticae.When there were 25 spider mites per leaf,the content of MDA and the activity of SOD were about 3.6 times and 10 times than control,respectively.The activities of peroxidase(POD)and catalase(CAT)continued to increase with time,and both reached their peaks at 72 h after the damage of T.urticae.At the same time,there was a certain interaction between the damage time and damage density of T.urticae.The results indicated that strawberry leaves had a stress response to the damage of T.urticae by regulating the contents of H_(2)O_(2) and MDA and the changes in the activities of various defense enzymes in their bodies.

关 键 词：二斑叶螨 草莓 过氧化氢 丙二醛 防御酶活性 

分 类 号：Q965[生物学—昆虫学] S433[农业科学—农业昆虫与害虫防治]