水稻雄性不育突变体dtp1的细胞学分析与基因定位  

Cytological Analysis and Gene Mapping of Male Sterile Mutant dtp1 in Rice

作　　者：沈亚琦 蒋文翔 项圆圆 王联红 张秋云 刘家林 贺浩华[1] 胡丽芳[1] SHEN Yaqi;JIANG Wenxiang;XIANG Yuanyuan;WANG Lianhong;ZHANG Qiuyun;LIU Jialin;HE Haohua;HU Lifang(College of Agronomy,Jiangxi Agricultural University/Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education/Research Center of Super Rice Engineering and Technology,Jiangxi Province/Collaboration Center for Double Cropping Rice Modernization Production,Nanchang,Jiangxi 330045)

机构地区：[1]江西农业大学农学院/作物生理生态与遗传育种教育部重点实验室/江西省超级稻工程技术研究中心/双季稻现代化生产协同中心,江西南昌330045

出　　处：《核农学报》2022年第4期689-697,共9页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金项目(32060455);江西省杰出自然科学基金(2018ACB21025);江西省自然科学基金(20202BAB205007)。

摘　　要：本研究利用;Co-γ辐射诱变松香早粳种子获得一个稳定遗传的水稻雄性不育突变体dtp1,为了研究其不育的发生机制并克隆目的基因,对其进行形态学观察、细胞学分析和基因定位。结果表明,dtp1突变体能完成正常的营养生长,但花药瘦小呈浅黄色,属无花粉型雄性不育突变体。石蜡切片的分析结果发现dtp1突变体能进行正常的减数分裂,与野生型(WT)相比,在小孢子发育时期dtp1突变体绒毡层染色较浅、小孢子形状不规则,后期绒毡层异常膨大,小孢子不能形成花粉粒。遗传学分析结果表明dtp1突变体受单隐性核基因控制,通过图位克隆的方法将DTP1基因定位在7号染色体SYrbC7-2680809和SYrbC7-2832575之间,物理距离为151.7 kb,共包含14个开放阅读框。本研究为进一步进行DTP1基因的克隆与功能分析奠定了理论基础。A rice male sterility mutant dtp1(defective tapetum and pollen 1)was obtained by irradiation of Songxiangzaojing seeds with;Co-γirradiation.In order to study the mechanism of sterility and clone the target gene the morphological observation,cytological analysis and gene mapping were carried out.The results showed that the dtp1 mutant had normal vegetative growth,but its anthers were thin and pale yellow,showing non-pollen-type male sterile.Paraffin section analysis showed that the dtp1 mutant could undergo normal meiosis,but the tapetum was slightly stained with irregular microspore at microspore development stage.In the later stage,the tapetum was abnormally enlarged and the microspore could not form pollen grains.Genetic analysis showed that dtp1 was controlled by a single recessive nuclear gene.By map-based cloning,the DTP1 gene was located between SYrbC7-2680809 and SYrbC7-2832575 on chromosome 7 with a physical distance of 151.7 kb containing 14 open reading frames.This study laid a foundation for further cloning and functional analysis of DTP1 gene.

关 键 词：水稻 雄性不育 dtp1 绒毡层 小孢子 

分 类 号：S511[农业科学—作物学]