提高肺炎克雷伯氏菌产1,3-丙二醇的分子生物学方法研究进展  

Molecular biological methods for improving the production of 1,3-propanediol by Klebsiella pneumonia:a review

作　　者：王世伟[1] 王卿惠 向文胜[2] 郝健[3] 李婷婷[1] 赵晨晨 周智博 WANG Shiwei;WANG Qinghui;XIANG Wensheng;HAO Jian;LI Tingting;ZHAO Chenchen;ZHOU Zhibo(Key Laboratory of Resistance Gene Engineering and Preservation of Biodiversity in Cold Areas,College of Life Sciences and Agriculture and Forestry,Qiqihar University,Qiqihar 161005,Heilongjiang,China;School of Life Science,Northeast Agricultural University,Harbin 150030,Heilongjiang,China;Lab of Biorefinery,Shanghai Advanced Research Institute,Chinese Academy of Sciences,Shanghai 201210,China)

机构地区：[1]齐齐哈尔大学生命与农林学院,抗性基因工程与寒地生物多样性保护重点实验室,黑龙江齐齐哈尔161005 [2]东北农业大学生命科学学院,黑龙江哈尔滨150030 [3]中国科学院上海高等研究院生物精炼实验室,上海201210

出　　处：《微生物学通报》2022年第6期2362-2377,共16页Microbiology China

基　　金：2021年度黑龙江省大学生创新创业训练计划项目(202110232101)。

摘　　要：1,3-丙二醇(1,3-propanediol,1,3-PD)可用于工业合成多种化合物,包括聚酯、聚醚和聚氨酯。发酵法生产1,3-PD具有巨大潜力。本文从代谢途径分析入手,梳理了肺炎克雷伯氏菌厌氧代谢途径的相关酶及催化作用,较详细地综述了其产1,3-PD关键酶的分子改造、基因工程菌株的构建和关键酶基因表达、副产物相关代谢酶基因敲除等方面的最新进展,并展望了其今后的发展前景。1,3-propanediol(1,3-PD)can be used for chemical synthesis of a variety of compounds,including polyesters,polyethers and polyurethanes.Fermentation has great potential to improve 1,3-PD production.This study summarized the enzymes in the anaerobic metabolic pathways of Klebsiella pneumoniae and the catalysis,and reviewed the molecular modification of key enzymes in 1,3-PD production of K.pneumoniae,construction of genetically engineered strains and the expression of key enzyme genes,and knockout of by-product-related metabolic enzyme genes.Moreover,we also described the development prospects of 1,3-PD.

关 键 词：肺炎克雷伯氏菌 1 3-丙二醇 分子生物学 方法 进展 

分 类 号：Q78[生物学—分子生物学] TQ223.162[化学工程—有机化工]