益气小复方通过下调lncRNA HCP5增强顺铂对三阴性乳腺癌的抑制作用  

Yiqi Formula enhance inhibiting effect of cisplatin on triple-negative breast cancer by down regulating lncRNA HCP5

作　　者：吴晶晶[1] 孟畑 马丽娜 周悦[1] 殷玉莲[1] 陈红风[1] 陈豪[1] WU Jingjing;MENG Tian;MA Lina;ZHOU Yue;YIN Yulian;CHEN Hongfeng;CHEN hao(Breast Department of Traditional Chinese Medicine,Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China)

机构地区：[1]上海中医药大学附属龙华医院中医乳腺科,上海200032

出　　处：《上海中医药大学学报》2022年第3期37-44,共8页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81704074);上海市名老中医药学术经验研究工作室建设项目(SHGZS-2017018);龙华医院陈红风名中医工作室建设项目。

摘　　要：目的:探讨益气小复方增强顺铂对三阴性乳腺癌抑制作用的机制。方法:①利用TCGA数据库,比较长链非编码RNA(lncRNA)HCP5在乳腺癌、三阴性乳腺癌肿瘤及瘤旁组织中的表达水平;②检测lncRNA HCP5,PTEN、p-Akt蛋白在三阴性乳腺癌肿瘤、瘤旁及非三阴性乳腺癌肿瘤组织中的表达水平;③构建三阴性乳腺癌裸鼠移植瘤模型,予以顺铂、益气小复方+顺铂干预,检测肿瘤体积、质量以及瘤体lncRNA HCP5,PTEN、p-Akt蛋白表达水平。结果:①TCGA数据库中,lncRNA HCP5在乳腺癌肿瘤组织中的表达水平高于瘤旁组织,但差异无统计学意义(P>0.05);Basal-like亚型乳腺癌肿瘤组织中lncRNA HCP5表达水平高于瘤旁组织,差异有统计学意义(P<0.01)。②三阴性乳腺癌肿瘤组织中lncRNA HCP5表达水平高于瘤旁及非三阴性乳腺癌组织(P<0.01),PTEN蛋白表达水平低于瘤旁及非三阴性乳腺癌组织(P<0.001),p-Akt蛋白表达水平高于瘤旁及非三阴性乳腺癌组织(P<0.001);③与顺铂组相比,益气小复方+顺铂组裸鼠移植瘤体积明显较小、质量较轻(P<0.001),瘤体lncRNA HCP5和p-Akt蛋白表达水平降低(P<0.001),PTEN蛋白表达水平升高(P<0.001)。结论:益气小复方可能通过下调lncRNA HCP5的表达来上调PTEN和下调p-Akt蛋白的表达,从而增强顺铂对三阴性乳腺癌的抑制作用。Objective:To explore the mechanism of Yiqi Formula enhancing the inhibiting function of cisplatin on triple-negative breast cancer.Methods:(1)The expression levels of lncRNA HCP5 in breast cancer tumor tissues,triple-negative breast cancer tumor and tumor adjacent tissues were compared using TCGA database.(2)The expression levels of lncRNA HCP5,PTEN and p-Akt protein in triplenegative breast cancer tumor and tumor adjacent tissues and non-triple-negative breast cancer tumor tissues were detected.(3)The xenograft model of nude mice with triple-negative breast cancer was established and treated with cisplatin,Yiqi Formula + cisplatin. Tumor volume,weight and expression levels of lncRNA HCP5,PTEN and P-Akt protein in the tumor were measured.Results:(1) The data of TCGA showed that the expression level of lncRNA HCP5 in breast cancer tumor tissues was higher than that in tumor adjacent tissues,but the difference was not statistically significant(P>0.05). In Basal-like breast cancer,the expression level of lncRNA HCP5 in tumor tissues was significantly higher than that in tumor adjacent tissues(P<0.01).(2)In triple-negative breast cancer tumor tissues,the expression level of lncRNA HCP5 was higher than that in tumor adjacent tissues and non-triple-negative breast cancer tumor tissues(P<0.01), the expression level of PTEN protein was lower than that in tumor adjacent tissues and non-triple-negative breast cancer tumor tissues(P<0.001),and the expression level of p-Akt protein was higher than that in tumor adjacent tissues and non-triple-negative breast cancer tumor tissues(P<0.001).(3) Compared with cisplatin group,the tumor volume and weight of Yiqi Formula + cisplatin group was significantly smaller and lighter(P<0.001),the expression level of lncRNA HCP5 and p-Akt protein decreased(P<0.001),the expression level of PTEN protein increased(P<0.001).Conclusion:Yiqi Formula may increase PTEN protein expression and decrease p-Akt protein expression by down regulating lncRNA HCP5,so as to enhance inhibiting effect of cispl

关 键 词：益气小复方 顺铂 三阴性乳腺癌 lncRNA HCP5 PTEN/p-Akt 

分 类 号：R285.5[医药卫生—中药学]