A broadly neutralizing human monoclonal antibody against the hemagglutinin of avian influenza virus H7N9  

作　　者：Jingxin Li Li Zhang Linlin Bao Yuxiao Wang Lin Qiu Jialei Hu Rong Tang Huiyan Yu Jun Shan Yan Li Chuan Qin Fengcai Zhu 

机构地区：[1]Department for Vaccine Clinical Evaluation,NHC Key Laboratory of Enteric Pathogenic Microbiology,Jlangsu Provincial Center for Diseases Control and Prevention,Nanjing,Jiangsu 210009,China [2]Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences(CAMS)&Comparative Medicine Center,Peking Union Medical Collage(PUMC),Key Laboratory of Human Disease Comparative Medicine,Ministry of Health,Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases,Beijing 100101,China [3]Department of Public Health,Southeast University,Nanjing,Jiangsu 210009,China [4]Shanghai Institute for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China

出　　处：《Chinese Medical Journal》2022年第7期799-805,共7页中华医学杂志（英文版）

基　　金：National Natural Science Foundation for Youth,China(No.81501793)。

摘　　要：Background:The new emerging avian influenza A H7N9 virus,causing severe human infection with a mortality rate of around 41%.This study aims to provide a novel treatment option for the prevention and control of H7N9.Methods:H7 hemagglutinin(HA)-specific B cells were isolated from peripheral blood plasma cells of the patients previously infected by H7N9 in Jiangsu Province,China.The human monoclonal antibodies(mAbs)were generated by amplification and cloning of these HA-specific B cells.First,all human mAbs were screened for binding activity by enzyme-linked immunosorbent assay.Then,those mAbs,exhibiting potent affinity to recognize H7 HAs were further evaluated by hemagglutination-inhibiting(HAI)and microneutralizationin vitro assays.Finally,the lead mAb candidate was selected and tested against the lethal challenge of the H7N9 virus using murine models.Results:The mAb 6-137 was able to recognize a panel of H7 HAs with high affinity but not HA of other subtypes,including H1N1 and H3N2.The mAb 6-137 can efficiently inhibit the HA activity in the inactivated H7N9 virus and neutralize 100 tissue culture infectious dose 50(TCID_(50))of H7N9 virus(influenza A/Nanjing/1/2013)invitro,with neutralizing activity as low as 78 ng/mL.In addition,the mAb 6-137 protected the mice against the lethal challenge of H7N9 prophylactically and therapeutically.Conclusion:The mAb 6-137 could be an effective antibody as a prophylactic or therapeutic biological treatment for the H7N9 exposure or infection.

关 键 词：Avian influenza H7N9 Monoclonal antibody Neutralizing activity 

分 类 号：R392-33[医药卫生—免疫学]