吉林省主栽水稻品种抗瘟性评价及抗瘟基因型鉴定  

Rice blast resistance evaluation and identification of major blast resistance genes in main rice varieties in Jilin Province,China

作　　者：杨林浩 王瀚 赵丹 李泽豪 林品轩 李大勇 孙文献 YANG Linhao;WANG Han;ZHAO Dan;LI Zehao;LIN Pinxuan;LI Dayong;SUN Wenxian(College of Plant Protection,Jilin Agricultural University/Jilin Key Laboratory of Green Management on Crop Diseases and Pests,Changchun 130118,China;College of Plant Protection and the Ministry of Agriculture Key Laboratory of Pest Monitoring and Green Management,China Agricultural University,Beijing 100193,China)

机构地区：[1]吉林农业大学植物保护学院/吉林省作物病虫害绿色防控重点实验室,长春130118 [2]中国农业大学植物保护学院/农业农村部作物有害生物监测与绿色防控重点实验室,北京100193

出　　处：《植物病理学报》2022年第3期404-415,共12页Acta Phytopathologica Sinica

基　　金：国家自然科学基金区域创新发展联合基金(U19A2027);国家自然基金青年科学基金项目(32001857);北京市自然科学基金重点项目(6181001);吉林省技术攻关项目(20190303002SF);吉林省教育厅科技项目(JJKH20200344KJ和JJKH20200348KJ);国家级大学生创新创业训练计划项目(202010193015)。

摘　　要：为明确吉林省主栽水稻品种的抗瘟性和抗瘟基因型,选用了一套已明确其无毒基因组成的稻瘟病菌标准菌株对吉林省68个主栽水稻品种进行喷雾和离体划伤接种试验。结果表明,68个品种的抗瘟能力存在较大差异,中抗以上抗病品种达到44个,占供试品种的64.7%;抗瘟基因推导结果结合特异性分子标记检测表明,Pita、Pia、Pish、Pita2、Pib和Pi9等6个基因为吉林省主栽水稻品种中主要的抗瘟基因,并且品种中含有的抗瘟基因数量与抗瘟能力呈正相关;根据结果推测,测试的所有品种中可能不含有Pi2、Pikh、Pikm、Pi11和Pi12基因。本研究揭示了吉林省近年主栽水稻品种的抗瘟能力和抗瘟基因组成,明确了吉林稻区主效抗瘟基因和基因聚合对稻瘟病的抗性贡献,为进一步培育广谱持久抗瘟品种和合理布局抗病品种提供了重要参考。In order to clarify the rice blast resistance and resistance genotypes of rice varieties that have been mainly planted in Jilin Province in recent years,a set of 25 standard strains of Magnaporthe oryzae with the identified avirulence genes were used for spray and wound inoculation.The results demonstrated that 68 rice varie-ties exhibited significant differences in blast resistance,and 44 varieties displayed moderate or high resistance,accounting for 64.7%of tested varieties.Postulation of blast resistance genes together with gene-specific molecular marker identification demonstrated that Pita,Pia,Pish,Pita2,Pib and Pi9 were the major blast resistance genes in Jilin Province.The number of blast resistance genes contained in the variety is positively correlated with blast resistance.Based on the results,none of these varieties contains Pi2,Pikh,Pikm,Pi11 or Pi12 genes.This study not only evaluated blast resistance and revealed blast resistance gene compositions of main rice varieties in Jilin Province but also clarified the contribution to blast resistance of major blast resistance genes and gene pyramiding in rice-growing region of Jilin.The results provide important references for breeding of broad-spectrum and durable blast resistance varieties and rational deployment of resistant varieties for the management of rice blast disease.

关 键 词：稻瘟病 水稻品种 抗性评价 抗瘟基因 基因聚合 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]