首次发现巴西果胶杆菌(Pectobacterium brasiliense)引起甘薯茎腐病  

First report of Pectobacterium brasiliense causing stem rot disease of sweet potato

作　　者：马媛媛 罗金燕[2] 王艳丽[3] 朱洁 李斌[1] 安千里[1] MA Yuanyuan;LUO Jinyan;WANG Yanli;ZHU Jie;LI Bin;AN Qianli(Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects,Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province,Institute of Biotechnology,Zhejiang University,Hangzhou 310058,China;Shanghai Agriculture Technology Extension&Service Center,Shanghai 201103,China;Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;Wenzhou Station of Plant Protection,Soils and Fertilizers,Wenzhou 325000,China)

机构地区：[1]浙江大学生物技术研究所,农业部作物病虫分子生物学重点实验室,浙江省作物病虫生物学重点实验室,杭州310058 [2]上海市农业技术推广服务中心,上海201103 [3]浙江省农业科学院植物保护与微生物研究所,杭州310021 [4]温州市植物保护与土壤肥料管理站,温州325000

出　　处：《植物病理学报》2022年第3期504-507,共4页Acta Phytopathologica Sinica

基　　金：浙江省重点研发项目(2017C02002);浙江省自然科学基金(LY19C010007)。

摘　　要：Dickeya和Pectobacterium属细菌的植物寄主广泛,包括十字花科、茄科、百合科、伞形花科和禾本科等科的农作物[1],所引发的细菌性软腐病严重影响农作物的产量和品质,造成严重经济损失,威胁农业生产和粮食安全。Dickeya和Pectobacterium属不同种的菌株能侵染同一种植物,产生相似的病症。如已发现能侵染马铃薯并导致软腐病害的有D.solani、D.dianthicola、D.dadantii、D.chrysanthemi、D.zeae、P.versatile、P.carotovorum、P.brasiliense、P.atrosepticum、P.betavasculorum、P.parmentieri、P.parvum、P.peruviense、P.polaris、P.actinidiae和P.punjabense等种的细菌[2-3]。又如P.carotovorum和P.odoriferum引发甘薯根腐病[4],D.dadantii引发甘薯茎腐病[5-7].The bacterial stem and root rot disease of sweet potato occurs in some regions of China and causes great economic losses.Dickeya dadantii is the known pathogen of this disease.We isolated pectin-degrading bacteria from diseased sweet potato plants grown in Jinhua,Zhejiang Province using the crystal violet pectate(CVP)medium.Inoculation of these bacterial isolates into stems of sweet potato seedlings caused weak symptoms of the stem rot disease.These bacterial isolates were identified as Pectobacterium brasiliense by multi-locus sequence(16 S rRNA,dnaX,leuS and recA genes)analysis and PCR amplification using P.brasiliense-specific primers.Inoculation of this P.brasiliense pathogen caused weak potato blackleg disease.This is the first report of P.brasiliense causing the stem rot disease of sweet potato in the field.

关 键 词：细菌性软腐病 甘薯茎腐病 甘薯根腐病 伞形花科 植物寄主 粮食安全 

分 类 号：S435.311[农业科学—农业昆虫与害虫防治]