大白菜LACS家族基因鉴定与表达分析  

Identification and Expression Analysis of LACS Family Genes in Chinese Cabbage (Brassica rapa L. ssp. pekinensis)

作　　者：王荣花 王树彬 刘栓桃[1] 张志刚[1] 李巧云[1] 王立华[1] 赵智中[1] Wang Ronghua;Wang Shubin;Liu Shuantao;Zhang Zhigang;Li Qiaoyun;Wang Lihua;Zhao Zhizhong(Vegetable Institute,Shandong Academy of Agricultural Sciences/Shandong Branch of National Center for Vegetables Improvement/Shandong Key Laboratory for Biology of Greenhouse Vegetables/Huanghe￣Huaihe Region Scientific Observation and Experim ntal Station of Vegetables,Ministry of Agriculture and Rural Affairs(Shandong),Jinan 250100,China)

机构地区：[1]山东省农业科学院蔬菜研究所/国家蔬菜改良中心山东分中心/山东省设施蔬菜生物学重点实验室/农业部黄淮地区蔬菜科学观测实验站(山东),山东济南250100

出　　处：《山东农业科学》2022年第6期1-9,共9页Shandong Agricultural Sciences

基　　金：山东省自然科学基金项目(ZR2019BC048);山东省农业良种工程项目(2019LZGC006,2019LZGC01703)。

摘　　要：长链脂酰辅酶A合成酶(LACS)能催化游离的脂肪酸形成酰基辅酶A,是脂肪酸激活、转运、氧化及储存脂合成等生化途径的关键酶,在许多生物过程中发挥重要作用。本研究利用生物信息学方法鉴定大白菜LACS家族基因成员,分析其亲缘关系、蛋白质理化性质、染色体定位、共线性关系、基因结构、蛋白保守结构域和基因表达模式。结果表明,从大白菜全基因组共鉴定到13个BrLACS基因成员,分成4组,不均匀地分布在7条染色体和1个Scaffold上。BrLACSs的蛋白长度和分子量分别为199~1 072 aa和23.10~121.10 kDa。与拟南芥AtLACS1、AtLACS5和AtLACS6共线性的BrLACS基因发生了扩张,出现了双拷贝(BrLACS5a、BrLACS5b和BrLACS6a、BrLACS6b)及三拷贝(BrLACS1a、BrLACS1b和BrLACS1c)。表达模式分析表明,BrLACS4和BrLACS8基因在大白菜不同器官/组织中表达量最高,尤其在控制花器官蜡质性状中起着重要作用,在有蜡粉大白菜中的表达量显著高于无蜡粉大白菜。本研究结果为解析大白菜LACS基因在控制大白菜蜡质性状中的分子机制和指导大白菜亮绿种质资源创新具有重要意义。Long chain fatty acyl CoA synthase(LACS) can catalyze free fatty acids to form acyl CoA, which is a key enzyme in biochemical pathways such as fatty acid activation, transport, oxidation, storage and lipid synthesis and plays an important role in many biological processes. In this study, the members of Chinese cabbage LACS genes were identified by bioinformatics method, and their phylogenetic relationship, physical and chemical properties of protein, chromosomal location, collinear relationship, gene structure, protein conserved domain and gene expression pattern were analyzed. The results showed that a total of 13 BrLACS genes were identified from the whole genome of Chinese cabbage. Phylogenetic analysis indicated that BrLACS genes were divided into 4 subgroups, which were unevenly distributed on 7 chromosomes and 1 Scaffold. The length and molecular weight of BrLACS proteins ranges from 199 to 1 072 aa and 23.10~121.10 kDa, respectively. BrLACS gene that were collinear with AtLACS1, AtLACS5 and AtLACS6 in Arabidopsis had been expanded, resulting in two copies(BrLACS5 a, BrLACS5 b and BrLACS6 a, BrLACS6 b) and three copies(BrLACS1 a, BrLACS1 b and BrLACS1 c). Expression analysis showed that BrLACS4 and BrLACS8 genes played important roles in controlling waxy trait in different organs/tissues of Chinese cabbage, especially in flower organs, and their expression levels in Chinese cabbage with waxy were significantly higher than Chinese cabbage with glossy. It was of great significance to analyzing the molecular mechanism of LACS genes of Chinese cabbage in controlling waxy traits and guiding the innovation of Chinese cabbage glossy germplasms.

关 键 词：大白菜 LACS家族 蜡质 生物信息学分析 基因表达 

分 类 号：S634.1[农业科学—蔬菜学] Q786[农业科学—园艺学]