参枣健脑口服液促进神经干细胞向神经元分化的作用机制研究  

Study on the Mechanism of Shenzao Jiannao Oral Liquid Promoting the Differentiation of Neural Stem Cells into Neurons

作　　者：李贺[1] 肖洪贺 陈吉聪 赵宇萌 田雨 田晋明 杨静娴[1] LI He;XIAO Hong-he;CHEN Ji-cong;ZHAO Yu-meng;TIAN Yu;TIAN Jin-ming;YANG Jing-xian(Department of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China)

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《现代中药研究与实践》2022年第3期25-30,共6页Research and Practice on Chinese Medicines

基　　金：辽宁省教育厅重点项目(L201945);辽宁省教育厅青年项目(L202036)。

摘　　要：目的探究参枣健脑口服液(SZJN)对神经干细胞(NSCs)分化的影响及其作用机制。方法原代培养海马NSCs,构建阿尔茨海默病(AD)细胞模型。CCK-8法检测AD细胞模型(APP-NSCs)细胞活力,微量酶标法检测乳酸脱氢酶释放量,神经球形成实验检测APP-NSCs细胞增殖能力,免疫荧光染色检测APP-NSCs细胞分化情况,NSCs迁移实验检测神经元迁移距离,Western blot法检测Wnt/β-catenin通路关键蛋白的表达情况。结果SZJN能够有效提高APP-NSCs细胞活力,减轻APP-NSCs损伤,增加神经球直径和神经球数量,促进NSCs和APP-NSCs向神经元方向分化,增加NSCs和APP-NSCs神经元迁移距离,提高Wnt-3a和β-catenin蛋白表达量,促进GSK-3β磷酸化。结论SZJN可通过激活Wnt/β-catenin信号通路促进NSCs向神经元分化。Objective To study the effect of Shenzao Jiannao oral liquid(SZJN)on the differentiation of neural stem cells(NSCs),and explore the underlying mechanism.Methods Primary culture of hippocampal neural stem cells to construct a model of Alzheimer’s disease cells.The activity of APP-NSCs cells is detected by CCK-8 assays,the level of lactic dehydrogenase in the culture is tested to assess the damage of APP-NSCs cell,immunofluorescence is used to detect the differentiation of APP-NSCs,the migration distances of neurons are measured to evaluate the migration of NSCs,Western blot analysis is used to detect the expression of key proteins in the Wnt/β-catenin pathway.Results SZJN can effectively improve the cell viability of APP-NSCs,alleviate the injury of APP-NSCs,increase the diameter and number of neurospheres,promote the neuronal differentiation of NSCs and APP-NSCs,and increase the migration distance of NSCs and APP-NSCs.Moreover,SZJN can also increase the protein levels of Wnt-3a andβ-catenin in neurons derived from APP-NSCs,as well as promote the phosphorylation of GSK-3β.Conclusion SZJN can promote the proliferation of APP-NSCs,as well as the neuronal differentiation of APP-NSCs by activating the Wnt/β-catenin signaling pathway.

关 键 词：参枣健脑口服液 阿尔茨海默病 神经干细胞 WNT/Β-CATENIN信号通路 

分 类 号：R285.5[医药卫生—中药学]