高效液相色谱法测定橙汁中酸性大红GR的不确定度评定  

Evaluation of uncertainty in the determination of acid scarlet GR in orange juice by high performance liquid chromatography

作　　者：袁圆 曾辉 肖艳 杨丽霞 崔晓娇 杨韵 吴后吕 YUAN Yuan;ZENG Hui;XIAO Yan;YANG Li-xia;CUI Xiao-jiao;YANG Yun;WU Hou-lu(Changsha Institute for Food and Drug Control,Changsha,Hunan 410036,China;National Alcohol Products Quality Inspection and Testing Center,Changsha,Hunan 410036,China;Hunan International Travel Heal Thcare Center,Changsha,Hunan 410011,China)

机构地区：[1]长沙市食品药品检验所,湖南长沙410036 [2]国家酒类产品质量检验检测中心[湖南],湖南长沙410036 [3]湖南国际旅行卫生保健中心[长沙海关口岸门诊部],湖南长沙410011

出　　处：《食品与机械》2022年第6期88-92,167,共6页Food and Machinery

基　　金：国家市场监督管理总局科技计划项目(编号:2020MK074)。

摘　　要：目的:对高效液相色谱法测定橙汁中酸性大红GR的含量进行不确定度评定,分析不确定度来源和影响检测结果的关键因素,从而提高检验结果的准确性。方法:建立橙汁中酸性大红GR含量不确定度的数学模型,对检测过程中可能引入不确定度的因素进行分析,并对各不确定度分量进行评定。结果:自制阳性橙汁中酸性大红GR含量为1.46mg/kg,扩展不确定度为0.11mg/kg(包含因子k=2),测定结果表示为:X=(1.46±0.11)mg/kg(k=2)。结论:不确定度主要来源于标准物质的纯度、标准曲线线性拟合和高效液相色谱仪分析所引入的不确定度。Objective:The uncertainty of the determination of acid scarlet GR in orange juice by high performance liquid chromatography(HPLC)was evaluated.The source of uncertainty and the key factors affecting the test results were analyzed,so as to improve the accuracy of the test results.Methods:The mathematical model of uncertainty of acid scarlet GR content in orange juice was established.The factors that may introduce uncertainty in the detection process were analyzed,and the uncertainty components were evaluated.Results:The content of acid scarlet GR in self-made positive orange juice was 1.46 mg/kg,the expanded uncertainty was 0.11 mg/kg(including factor k=2),and the determination result was expressed as:X=(1.46±0.11)mg/kg(k=2).Conclusion:This uncertainty mainly comes from the uncertainty introduced by the purity of reference materials,the linear fitting of standard curves and the analysis of high performance liquid chromatography.

关 键 词：高效液相色谱 橙汁 酸性大红GR 不确定度 

分 类 号：O657.72[理学—分析化学] TS255.44[理学—化学]