8种材质上血痕中去痛片及其代谢物GC-MS/MS法测定的比较  

Detection of somedon and its metabolites in the dried blood spots on different materials by GC-MS/MS

作　　者：杨睿 禹明筠 王凌霄 张潮 贾娟 曹洁 崔海燕 贠克明 尉志文 Yang Rui;Yu Mingjun;Wang Lingxiao;Zhang Chao;Jia Juan;Cao Jie;Cui Haiyan;Yun Keming;Wei Zhiwen(College of Forensic Medicine,Shanxi Medical University,Jinzhong,Shanxi,030600,China;Key Laboratory of Forensic Toxicology,Ministry of Public Security,Jinzhong,Shanxi,030600,China)

机构地区：[1]山西医科大学法医学院法医毒物分析教研室,山西晋中030600 [2]法庭毒物分析公安部重点实验室,山西晋中030600

出　　处：《中国法医学杂志》2022年第3期251-254,260,共5页Chinese Journal of Forensic Medicine

基　　金：国家重点研发计划项目资助(2017YFC0803504,2018YFC0807403)。

摘　　要：目的对不同载体干血痕中去痛片主要成分及其代谢物提取方法进行比较,为疑似去痛片中毒案件现场中干血痕提取及检测提供实验依据。方法对载体为大理石、玻璃、光滑金属、木材、有漆木材、塑料、皮革、有漆金属8种材料采用棉签、纱布和棉布三种不同提取介质,按照擦拭40、60、80次干血痕后,置于离心管加水,先后调酸碱并以乙酸乙酯两次萃取合并挥干,甲醇复溶,按MRM模式使用GC-MS/MS检测去痛片主要成分及其代谢物。结果使用棉签擦拭60次并更换新的棉签重复2次为最优介质提取方法;不同干血痕载体材质对比中,水泥响应值最低、木材稍高,玻璃、金属及大理石响应值最高;案件应用中,血棉签与本试验方法的检验结果具有较好的相关性,相关系数最低为0.982,最高为>0.999。结论本研究建立提取方法及前处理方法可行性较好,可以支持法医学实践中的定性及部分定量检验。Objective Make a comparison of different extraction methods of somedon and its metabolites in the dried blood spots on different materials,It will provide an experimental basis for the somedon poisoning cases.Methods The dried blood spot on the smooth marble,glass,metal,wood,lacquer wood,plastic,leather and paint metal were extracted by cotton swabs,gauzes and cotton cloths,respectively.Each extraction tool scrubbed 40,60 and 80 times.Then the extraction tools were separately placed in centrifuge tubes.Water(pH=3~4)was added into the tubes and then ethyl acetate was used to extract.The samples were dried by nitrogen and analyzed by GC-MS/MS with the MRM mode.Results The optimal extraction method was that wiped 60 times by a cotton swab and repeated with new cotton swabs for two times.The response values of cement and wood were the lowest and the response values of glass,metal and marble were the highest among these materials.Extraction by cotton swabs had a good correlation,and the correlation coefficient were from 0.982 to 0.999.Conclusion In this study,the extraction and pretreatment methods are feasible,which can be used for the qualitative and partial quantitative tests in forensic medicine practice.

关 键 词：法医毒物分析 去痛片及其代谢物 串联气相色谱-质谱法 提取方法 载体材质 

分 类 号：D919.4[医药卫生—法医学]