白术与菊科混伪品的鉴别研究  

Study on Identification of Atractylodis Macrocephalae Rhizoma and Adulterants of Compositae

作　　者：李子璐 余国鑫 陈君 LI Zilu;YU Guoxin;CHEN Jun(Department of Pharmacognosy,School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 210009,China)

机构地区：[1]中国药科大学中药学院生药学系,南京210009

出　　处：《药学与临床研究》2022年第3期233-236,275,共5页Pharmaceutical and Clinical Research

基　　金：国家重点研发计划(2019YFC1711000)。

摘　　要：目的:对白术及几种菊科混伪品进行比较研究,为白术鉴别与质量控制提供有效方法。方法:采用性状及显微鉴别方法对白术及混伪品进行观察,并建立高效液相色谱指纹图谱方法,使用Agilent Eclipse Plus C18(4.6×250 mm,5μm)色谱柱;以0.05%磷酸-乙腈为流动相,梯度洗脱;柱温25℃;检测波长232 nm;流速1 mL·min-1;进样体积:10μL,对药材进行分析;采用液质联用技术对共有峰进行鉴定,结合相似度评价区分白术及混伪品。结果:白术和混伪品被成功鉴别,所建指纹图谱可正确区分白术与混伪品。结论:综合传统鉴别手段与建立的指纹图谱方法,可直观、多层次地甄别白术与混伪品间的差异,为白术的准确鉴定和质量控制提供可靠方法。Objective:To comparatively analyze Atractylodis Macrocephalae Rhizoma and adulterants of Compositae,provide an effective method for quality control of Atractylodis Macrocephalae Rhizoma.Methods:Besides the characteristic and microscopic identification to distinguish Atractylodis Macrocephalae Rhizoma and its adulterants,an HPLC fingerprint method was established.The separation was performed on an Agilent Eclipse Plus C18 column(4.6 mm×250 mm,5μm)with 0.05%phosphoric acid-acetonitrile as mobile phase.The flow rate was 1 mL·min-1,the column temperature was 25℃,the injection volume was 10μL,and the detection wavelength was 232 nm.The common peaks were identified by high performance liquid chromatography-quadrupole time of flight mass spectrometry.The similarity evaluation was combined to compare Atractylodis Macrocephalae Rhizoma and its adulterants.Results:Atractylodis Macrocephalae Rhizoma and adulterants were distinguished through observation and HPLC fingerprint analysis.Conclusion:Combining traditional identification methods and established fingerprint method,the differences between Atractylodis Macrocephalae Rhizoma and adulterants can be visually and by multi-level displayed,which can provide a reliable method for accurate identification and quality control of Atractylodis Macrocephalae Rhizoma.

关 键 词：白术 混伪品 鉴别 高效液相色谱 指纹图谱 

分 类 号：R927[医药卫生—药学]