P53对基于MRI的骨关节炎间隙狭窄程度影响的临床研究  

Clinical study of the effect of P53 on the degree of osteoarthritis space stenosis based on magnetic resonance imaging

作　　者：张双[1] 信瑞强[1] 石逸杰[1] 李艳翠[1] 彭如臣[1] ZHANG Shuang;XIN Ruiqiang;SHI Yijie;LI Yancui;PENG Ruchen(Department of Radiology,Beijing Luhe Hospital,Capital Medical University,Beijing 101149,China)

机构地区：[1]首都医科大学附属北京潞河医院放射科,北京101149

出　　处：《磁共振成像》2022年第6期122-126,共5页Chinese Journal of Magnetic Resonance Imaging

摘　　要：目的利用生物信息学技术对骨关节炎(osteoarthritis,OA)差异表达基因进行识别和功能富集分析,并通过临床数据研究P53对基于MRI的OA间隙狭窄程度的影响。材料与方法下载微阵列数据(GSE55235),利用GEO2R鉴别OA患者与正常滑膜样本间的差异表达基因(differentially expressed genes,DEGs)。利用基因本体论(gene ontology,GO)和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)对差异表达基因进行基因富集分析,进一步构建蛋白—蛋白互作(protein-protein interaction,PPI)网络分析,识别重要模块,鉴定核心基因。选取50例OA间隙狭窄患者,利用MRI检测其骨关节情况,分析其临床资料,运用Pearson卡方检验、Spearman相关性分析和多因素Logistic回归进行统计学分析。结果对照组与OA组滑膜组织之间存在差异。GSE55235的分析结果识别出91个差异表达基因。DEGs集富集分析全面概述了OA的一些主要病理生理机制:细胞对过氧化氢的反应、P53信号通路。鉴定出了4个关键基因:MMP1、CXCL8、VEGFA和JUN。与正常组对比,MMP1在OA组中高表达,CXCL8、VEGFA和JUN在OA组中低表达。Pearson卡方检验、Spearman相关性分析和多因素Logistic回归结果显示P53与OA间隙狭窄程度显著相关(OR=0.112,95%CI:0.025~0.495,P=0.004),而性别、年龄、MMP1、VEGFA、CXCL8与OA间隙狭窄程度无显著相关性(P值均大于0.05)。结论基于MRI结果的基础上,判断细胞对过氧化氢的反应、P53信号通路能为OA的诊断和治疗提供新的研究靶点。P53在OA患者中低表达,可能作为其潜在治疗靶点。MRI也有利于OA患者早期的诊断与治疗,降低OA发病率。Objective:To identify and analyze the differentially expressed genes in osteoarthritis(OA)using bioinformatics techniques,and clinical data were used to study the effect of P53 on the degree of OA gap stenosis based on MRI.Materials and Methods:Microarray data(GSE55235)were downloaded and GEO2R was used to identify differentially expressed genes(DEGs)between OA patients and normal synovial samples.The enrichment analysis for the differentially expressed genes was performed by using the gene ontology(GO)and the Kyoto Encyclopedia of Genes and Genomes(KEGG).Protein-protein interaction network(PPI)analysis was further constructed to identify important modules and identify core genes.Fifty patients with OA space stenosis were selected,and their bone and joint conditions were detected by MRI,and their clinical data were analyzed by Pearson Chi-square test,Spearman correlation analysis and multivariate Logistic regression.Results:The difference of synovial tissue between control group and OA group was analyzed.Analysis of GSE55235 identified 91 differentially expressed genes.DEGs enrichment analysis comprehensively summarized some main pathophysiological mechanisms of OA:cell response to hydrogen peroxide,P53 signaling pathway.Four hub genes were identified:MMP1,CXCL8,VEGFA and JUN.Compared with the normal group,MMP1was highly expressed in the OA group,while CXCL8,VEGFA and JUN were down-regulated in the OA group.Pearson Chi-square test,Spearman correlation analysis and multiple Logistic regression results showed that P53 was significantly correlated with the degree of OA space stenosis(OR=0.112,95%CI:0.025-0.495,P=0.004),while gender,age,MMP1,VEGFA and CXCL8 were not significantly correlated with the degree of OA space stenosis(P values were all greater than 0.05).Conclusions:In conclusion,based on MRI results,it is concluded that cell reaction to hydrogen peroxide and P53 signaling pathway can provide new research targets for the diagnosis and treatment of OA.The low expression of P53 in OA patients may be a potentia

关 键 词：磁共振成像 骨关节炎 生物信息学 差异表达基因 富集分析 P53信号通路 

分 类 号：R445.2[医药卫生—影像医学与核医学] R684.3[医药卫生—诊断学]