猪蓝耳病病毒与副猪嗜血杆菌及猪链球菌混合感染的诊治  

Diagnosis and Treatment of the Co-infection of Porcine Reproductive and Respiratory Syndrome Virus,Haemophilus parasuis and Streptococcus suis

作　　者：许艳华 李富强[2,3] 董志民 田向学[2,3] 任卫科 路超[2,3] XU Yanhua;LI Fuqiang;DONG Zhimin;TIAN Xiangxue;REN Weike;LU Chao(Ninghe Husbandry and Aquaculture Development Services Center of Tianjin,Tianjin 301500,China;Animal Husbandry and Veterinary Research Institute,Tianjin Academy of Agricultural Sciences,Tianjin 300384,China;Tianjin Observation and Experiment Site of National Animal Health,Tianjin 300384,China)

机构地区：[1]天津市宁河区畜牧水产业发展服务中心,天津301500 [2]天津市农业科学院畜牧兽医研究所,天津300384 [3]国家动物疫病天津观测实验点,天津300384

出　　处：《天津农业科学》2022年第S01期22-27,共6页Tianjin Agricultural Sciences

基　　金：天津市生猪产业技术体系猪病毒病岗位项目(ITTPRS2021003);天津市科技支撑重点项目(19YFZCSN00570);中央级公益性科研院所基本科研业务费院级统筹项目。

摘　　要：为了确诊河北省唐山市某猪场保育猪发病原因,在进行临床调查的基础上,采集发病猪的肺脏、脾脏、淋巴结进行病原学检测。临床调查结果显示,发病猪出现发烧、喘、消瘦、腹泻、死亡等症状。实验室检测结果表明,5份病料均为PRRSV NADC30类似株阳性,核苷酸序列同源性分析结果表明其GP5蛋白基因与NADC30类似株的同源性最高为92.0%,与TJM株的同源性最低为84.3%。从发病猪肺脏和淋巴结中分离到副猪嗜血杆菌和猪链球菌,药敏试验结果显示,氟苯尼考、泰万菌素、林可霉素、强力霉素为中介。综合临床调查和实验室检测结果,确定引起该猪场保育猪发病的病因为PRRSV NADC30类似株和副猪嗜血杆菌、猪链球菌混合感染。提出在饲料中添加替米考星、腐殖酸钠及黄芪多糖,发病保育猪肌肉注射瑞可新的药物治疗方法,以及加强消毒的防控措施。猪场依法施治3周后无新发病例,7周后猪群生产稳定。综上,研究结果可为猪群病毒病细菌病混合感染的诊断和防治提供参考。To diagnose the cause of the disease of nursery pigs in a farm of Tangshan City,Hebei Province,based on the clinical investigation,the lungs,spleens,lymph nodes of the infected pigs were collected for pathogenic detection.The results of clinical investigation showed that the symptoms of diseased pigs were fever,asthma,thin,diarrhea,death and so on.The laboratory test results showed that the five samples were postive for Porcine Reproductive and Respiratory Syndrome Virus(PRRSV)NADC30-likes strain.The nucleotide sequence homology analysis result showed that the homology of GP5 protein gene between the pathogenic strain and NADC30 strain was the highest,92.0%,and homology of GP5 protein gene between the pathogenic strain and TJM strain was the lowest,84.3%.Haemophilus parasuis and Streptococcus suis were isolated from the lungs and lymph nodes of diseased pigs.The results of drug sensitivity test showed that florfenicol,tylvalosin,lincomycin and doxycycline were the drug intermediary.Based on the results of clinical investigation and laboratory test,it was determined that the disease of nursery pigs in the farm was caused by the co-infection with PRRSV NADC30-likes strain,Haemophilus parasuis and Streptococcus suis.It was proposed to add tilmicosin,sodium humate and astragalus polysaccharide to the feed,inject recosin into the muscle of sick and nursing pigs,and strengthen the prevention and control measures of disinfection.There were no new cases in the pig farm after 3 weeks of treatment according to law,and the production of pigs was stable after 7 weeks.In conclusion,the research results can provide reference for the diagnosis and prevention of mixed infection of virus disease and bacterial disease in pigs.

关 键 词：猪繁殖与呼吸综合征病毒 副猪嗜血杆菌 猪链球菌 混合感染 诊治 

分 类 号：S858.28[农业科学—临床兽医学]