白菜型冬油菜HDACs基因家族的鉴定及表达分析  

Identification and Expression Analysis of HDACs Gene Family in Winter Rapeseed(Brassica rapa L.)

作　　者：徐芳 刘丽君 李鹏 姚彦林 孙万仓[1,2] 武军艳 XU Fang;LIU Lijun;LI Peng;YAO Yanlin;SUN Wancang;WU Junyan(College of Agriculture, Gansu Agricultural University, Lanzhou 730070, China;2State Key Laboratory of Arid Habitat Crops, Lanzhou 730070, China)

机构地区：[1]甘肃农业大学农学院,兰州730070 [2]干旱生境作物学国家重点实验室,兰州730070

出　　处：《西北植物学报》2022年第5期721-735,共15页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31960435);财政部和农业农村部国家现代农业产业技术体系(CARS-12);甘肃农业大学科技创新基金-公招博士科研启动基金(GAU-KYQD-2018-42);省部共建干旱生境作物学国家重点实验室(甘肃农业大学)开放基金课题(GSCS-20202-08)。

摘　　要：为了研究HDACs(组蛋白去乙酰化酶)在白菜型冬油菜响应低温胁迫中的影响,该研究采用强抗寒品种‘陇油7号’、耐寒品种‘天油4号’和弱抗寒品种Lenox等白菜型冬油菜,分析HDACs抑制剂曲古抑菌素A(TSA)处理后白菜型冬油菜种子的萌发,及低温胁迫下抗氧化酶活性和渗透调节物质含量变化,并全基因组鉴定HDACs的基因家族,分析其在不同抗寒性白菜型冬油菜中的表达特性。结果表明:(1)1μmol/L和3μmol/L的TSA对3个品种的根长均具有抑制作用,不同温度下外源喷施TSA均可增加抗氧化酶活性和渗透调节物质含量。(2)在白菜型冬油菜中共鉴定出21个HDACs;系统发育分析显示,HDACs可分为3个亚家族,所有基因不均等分布在8条染色体上,但7号和8号染色体上没有基因分布;HDACs启动子序列中包含胁迫响应、光响应、激素应答等相关的作用元件。(3)组织表达结果显示,大部分基因在生长锥中表达量明显高于其他组织部位,BrapaHDA19、BrapaHDA6-1、BrapaHDA8和BrapaHDA7-2在白菜型冬油菜花中表达量较高,且BrapaHDA5-2在‘天油4号’的茎生叶、角果、新基叶和老基叶中的表达量较高,而BrapaHDA7-2在Lenox的花中表达量较高。(4)低温胁迫表达结果显示,BrapaHDA8、BrapaHDA14、BrapaHDA2、BrapaHDT2-2和BrapaHDA15-1在3个抗寒性不同的材料中呈现出差异表达。研究推测,所鉴定的这些基因可能在白菜型冬油菜生长发育及抗寒性方面发挥重要功能。In order to study the effects of HDACs(histone deacetylation)in winter rapeseed(Brassica rapa L.)under low temperature stress,with strong cold resistance varieties of‘Longyou 7’,low temperature resistance varieties of‘Tianyou 4’and weak cold resistance varieties of Lenox,winter rapeseed as materials,we analyzed HDACs inhibitor trichostatin A(TSA)postpartum seed germination,low temperature stress antioxidant enzyme activity and osmotic adjustment substances content changes,and identified the genetic family of HDACs in the genome and analyzed its expression characteristics in different cold winter rapeseed varieties.The results show that:(1)both 1 and 3μmol/L TSA have inhibit effect on the root length of three varieties,and the exogenous spray TSA at different temperatures increased antioxidant enzyme activities and osmotic adjustment substance contents.(2)In this study,a total of 21 HDACs members in winter rapeseed were identified,system development result showed that HDACs were divided into three sub-families,the chromosome location found that all gene were distributed unevenly on eight chromosomes,with no gene distribution on chromosomes 7 and 8.The HDACs promoter sequence contains many cis-acting elements that related to stress response,light response and hormones response.(3)The results of different tissue sites showed that most genes were significantly higher in shoot apical meristem than in other tissues.BrapaHDA19,BrapaHDA6-1,BrapaHDA8 and BrapaHDA7-2 were high in winter rapeseed,BrapaHDA5-2‘Tianyou 4’stem leaf,silique,basal leaf(new)and basal leaf(old)have higher expression,and BrapaHDA7-2 is high in lenox flowers.(4)Low temperature stress expression results showed that BrapaHDA8,BrapaHDA14,BrapaHDA2,BrapaHDT2-2 and BrapaHDA15-1 showed differentially expressed in three cold resistance different materials.Inference by the above results,these genes may play an important role in the growth and cold resistance in winter rapeseed.

关 键 词：白菜型冬油菜 TSA HDACs基因家族 组蛋白去乙酰化 低温胁迫 基因表达 

分 类 号：Q785[生物学—分子生物学] Q786