木薯UGT41基因对细菌性枯萎病的抗性研究  

Isolation and Preliminarily Functional Analysis of MeUGT41 Gene from Cassava

作　　者：曾坚 谢雅倩 李宝莹 张海涵 胡伟[2] ZENG Jian;XIE Yaqian;LI Baoying;ZHANG Haihan;HU Wei(Henry Fok College of Biology and Agriculture, Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern China, Shaoguan University, Shaoguan, Guangdong 512005, China;2Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou 571101, China)

机构地区：[1]韶关学院英东生物与农业学院,广东省粤北食药资源利用与保护重点实验室,广东韶关512005 [2]中国热带农业科学院热带生物技术研究所,海口571101

出　　处：《西北植物学报》2022年第5期736-741,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31901537);广东省基础与应用基础研究基金(2021A1515011236);广东省教育厅青年创新人才项目(2018KQNCX234);韶关学院博士启动项目(99000615)。

摘　　要：糖基转移酶广泛存在于植物中,其中UDP依赖型糖基转移酶(UDP-glycosyltransferases,UGTs)基因家族是糖基转移酶中的一大类。该研究以华南124木薯品种(Manihot esculenta cv.SC124)为材料,利用RT-PCR技术克隆木薯MeUGT41基因,以病毒诱导干扰木薯MeUGT41基因的表达量,并对基因干扰植株进行细菌性枯萎病抗性评价,为研究MeUGT41基因在木薯抵抗细菌性枯萎病的抗病机理奠定基础。结果表明:(1)地毯草黄单胞菌(Xamthomonas axonopodis pv.Manihotis,Xam)可显著诱导木薯MeUGT41基因表达。(2)成功构建MeUGT41的病毒诱导基因沉默(VIGS)载体,将干扰载体转化至木薯叶片进行MeUGT41基因沉默,荧光定量PCR检测结果显示,木薯叶片中MeUGT41基因的表达量显著下降。(3)Xam侵染实验表明,干扰抑制MeUGT41基因表达可显著降低木薯植株叶片对Xam病菌侵染的抵抗能力。研究认为,木薯叶片中MeUGT41基因具有抵抗Xam病菌侵染的能力。Cassava(Manihot esculenta Crantz)is an important food crop and cash crop in tropical and subtropical regions.UDP-glycosyltransferases(UGTs)belongs to glycosyltransferases,which is found in all plant species.In this research,Manihot esculenta cv.SC124 was regarded as material,and the MeUGT41 gene was cloned by RT-PCR and its function in cassava bacterial blight resistance was analyzed.These research provided clues for further study on the mechanism of MeUGT41 gene in cassava resistance to biotic stress.The results showed that:(1)the expression of MeUGT41 gene was significantly induced by Xamthomonas axonopodis pv.Manihotis(Xam).(2)Virus induced gene silencing(VIGS)vector of MeUGT41 gene was constructed.Through gene silencing in cassava leaves,qRT-PCR results showed that MeUGT41 gene was significantly decreased in cassava leaves.(3)Subsequently,Xam infection experiments were carried out on cassava leaves,and the results showed that the downregulation of MeUGT41 gene expression significantly reduced the resistance to Xam infection.

关 键 词：木薯 MeUGT基因 非生物胁迫 生物胁迫 VIGS Xam 

分 类 号：Q785[生物学—分子生物学] Q786[农业科学—作物学] S533