松叶猪毛菜NADP-苹果酸酶基因家族及启动子克隆分析  

Cloning and Analysis of NADP-ME Gene Family and Promoters in Salsola laricifolia

作　　者：张玉慧 王玉兰 夏春兰 闻志彬[1,2,3] ZHANG Yuhui;WANG Yulan;XIA Chunlan;WEN Zhibin(State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China;2The Specimen Museum of Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China;3University of Chinese Academy of Sciences, Beijing 100049, China)

机构地区：[1]中国科学院新疆生态与地理研究所,荒漠与绿洲生态国家重点实验室,乌鲁木齐830011 [2]中国科学院新疆生态与地理研究所标本馆,乌鲁木齐830011 [3]中国科学院大学,北京100049

出　　处：《西北植物学报》2022年第5期760-769,共10页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31970354,31670339);中国科学院青年创新促进会项目(2018479)。

摘　　要：NADP-苹果酸酶(NADP-ME)是C_(4)植物的关键光合酶,在生物和非生物胁迫中发挥了重要的作用。为了进一步研究该酶编码基因的功能,该研究以典型荒漠C_(3)-C_(4)中间型植物松叶猪毛菜为研究对象,在克隆得到NADP-ME家族基因序列的基础上,研究其表达部位及在非生物胁迫下的表达模式,并克隆其启动子序列分析响应非生物胁迫的元件差异。结果表明:(1)成功获得松叶猪毛菜3个NADP-MEs,命名为SaNADP-ME1、SaNADP-ME2和SaNADP-ME4,CDS序列长度分别为1755、1758和1941 bp。(2)SaNADP-ME1主要在根中表达,SaNADP-ME2和SaNADP-ME4主要在叶中表达;在ABA、NaCl、NAHCO_(3)和PEG_(6000)胁迫下松叶猪毛菜幼苗中3个NADP-MEs均可被诱导表达,且SaNADP-ME2和SaNADP-ME4的响应表达模式相似。(3)成功克隆得到SaNADP-ME1、SaNADP-ME2和SaNADP-ME4启动子区域2351、1655和2887 bp。生物信息学分析发现它们都含有基本启动子元件以及响应外界刺激的元件。NADP-malate enzyme(NADP-ME)is a key photosynthetic enzyme in C_(4) plants and plays an important role in biotic and abiotic stress.To further study the function of NADP-ME,we cloned the NADP-ME family gene sequences from the leaves of C_(3)-C_(4) species Salsola laricifolia,and then determined the expression patterns in tissues under different abiotic stresses.We also cloned NADP-MEs promoter sequences and analyzed the element differences in response to abiotic stress based on bioinformatics software.The results showed that:(1)three NADP-MEs were obtained,named SaNADP-ME1,SaNADP-ME2 and SaNADP-ME4,with the length of CDS sequence was 1755 bp,1758 bp and 1941 bp,respectively.(2)SaNADP-ME1 was mainly expressed in roots,while SaNADP-ME2 and SaNADP-ME4 were mainly expressed in leaves.Under the stress of ABA,NaCl,NaHCO_(3) and PEG_(6000),three NADP-MEs could be induced to express in Salsola laricifolia,and the response expression patterns of SaNADP-ME4 and SaNADP-ME2 were similar.(3)The length of promoter regions of SaNADP-ME1,SaNADP-ME2,and SaNADP-ME4 were 2351 bp,1655 bp and 2887 bp.Bioinformatics analysis showed that they contained not only basic promoter elements,but also elements in response to stress response.

关 键 词：NADP-ME家族 C_(4)基因表达 C_(3)-C_(4)中间型植物 松叶猪毛菜 

分 类 号：Q785[生物学—分子生物学] Q786