重度干旱胁迫下燕麦叶片活性氧清除系统对喷施腐植酸水溶肥的响应  

Response of Active Oxygen Scavenging System in Oat Leaves to Humic Acid Water-Soluble Fertilizer Application under Severe Drought Stress

作　　者：李英浩 刘景辉[1] 米俊珍[1] 吕品[1] 潘越 赵宝平[1] Allen Xue LI Yinghao;LIU Jinghui;MI Junzhen;LÜPin;PAN Yue;ZHAO Baoping;Allen Xue(Inner Mongolia Agricultural University,College of Agriculture,Hohhot 010019,China;Canadian Ministry of Agriculture and Agricultural Products Ottawa Research and Development Center,Ottawa,Canada KIA0C6.)

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019 [2]加拿大农业与农产品部渥太华研发中心,加拿大渥太华KIA0C6

出　　处：《西北植物学报》2022年第5期803-810,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家重点研发计划(2018YFE0107900);国家燕麦荞麦产业技术体系项目(CARS-08-B-5)。

摘　　要：以燕麦品种‘燕科2号’为试验材料,采用盆栽方式,设置正常供水(CK)、正常供水下喷施腐植酸水溶肥(CKH)、重度干旱胁迫(SS)和重度干旱胁迫下喷施腐植酸水溶肥(SSH)4个处理,对燕麦叶片中活性氧水平、抗氧化酶活性、总抗氧化剂含量及产量等进行测定,以明确腐植酸水溶肥(HA)对重度干旱胁迫下燕麦叶片活性氧清除系统的调控效应,并探讨HA对燕麦耐旱性的影响及其作用机制。结果表明:(1)与CK相比,燕麦叶片超氧阴离子(O^(-·)_(2))、羟自由基(·OH)、过氧化氢(H_(2)O_(2))和丙二醛(MDA)含量、以及超氧化物歧化酶(SOD)和过氧化物酶(POD)活性在重度干旱胁迫下显著提高,且均在喷施HA后比重度干旱胁迫处理显著降低,但此时活性氧的水平仍显著高于CK。(2)与CK相比,燕麦叶片过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、谷胱氨肽还原酶(GR)和谷胱氨肽过氧化物酶(GPX)活性在重度干旱胁迫下显著降低,而其总抗氧化能力(T-AOC)显著提高,它们在喷施HA后均比重度干旱胁迫处理显著提高,但各酶活性仍不同程度低于CK。(3)与CK相比,燕麦籽粒产量和生物产量在重度干旱胁迫下显著下降,喷施HA后又比重度干旱胁迫显著升高,但仍显著低于CK。研究认为,喷施HA可有效提高重度干旱胁迫下燕麦叶片抗氧化酶活性,促进抗氧化物质再生,增强叶片的总抗氧化能力,从而有效清除重度干旱胁迫引起的活性氧积累,降低重度干旱胁迫对植物细胞膜的氧化损伤,最终缓解重度干旱胁迫对燕麦造成的伤害,一定程度上能够减少籽粒产量的损失。Oat cultivar‘Yanke 2’was used as experimental material.Four treatments,namely normal water supply(CK),spraying humic acid soluble fertilizer(CKH)under normal water supply,severe drought stress(SS)and spraying humic acid soluble fertilizer(SSH)under severe drought stress,were set in pot.We determined reactive oxygen species(ROS)level,antioxidant enzyme activity,total antioxidant content and yield in oat leaves to clarify the regulation effect of humic acid water-soluble fertilizer(HA)on ROS scavenging system in oat leaves under severe drought stress,and to explore the effect of HA on drought tolerance of oat and its mechanism.The results show that:(1)compared with CK,the contents of superoxide anion(O^(-·)_(2)),hydroxyl radical(·OH),hydrogen peroxide(H_(2)O_(2))and malondialdehyde(MDA),as well as the activities of superoxide dismutase(SOD)and peroxidase(POD)in oat leaves were significantly increased under severe drought stress,and all of them were significantly decreased after spraying HA.However,the level of active oxygen was still significantly higher than CK(P<0.05).(2)Compared with CK,the activities of catalase(CAT),ascorbate peroxidase(APX),glutathione reductase(GR)and glutathione peroxidase(GPX)in oat leaves were significantly decreased under severe drought stress,while the total antioxidant capacity(T-AOC)was significantly increased after spraying HA,but the activity of each enzyme was still lower than CK(P<0.05).(3)Compared with CK,the grain yield and biological yield of oat were significantly decreased under severe drought stress,and significantly increased after spraying HA,but still significantly lower than CK(P<0.05).In conclusion,spraying HA under severe drought stress can effectively improve the antioxidant enzyme activity of oat leaves,promote renewable antioxidants,enhance the total antioxidant capacity,thereby effectively remove caused severe drought stress accumulation of reactive oxygen species,reduce oxidative damage of severe drought stress on plant cell membrane,eventually relieve sever

关 键 词：燕麦 干旱胁迫 腐植酸水溶肥 活性氧清除系统 

分 类 号：Q945.78[生物学—植物学] S512.6[农业科学—作物学]