甲状腺激素合成代谢与人体内脏脂肪米色化基因表达的相关性  

Correlation between thyroid hormone anabolism and beige gene expression in human of visceral fat

作　　者：蔡锦锋 俞静[2] 张文娜 朱浩 丁国宪[2] 王晓东[1] CAI Jinfeng;YU Jing;ZHANG Wenna;ZHU Hao;DING Guoxian;WANG Xiaodong(Department of Endocrinology,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China;Division of Geriatric Endocrinology,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China)

机构地区：[1]南京医科大学第一附属医院内分泌科,江苏南京210029 [2]南京医科大学第一附属医院老年内分泌科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2022年第5期679-683,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省卫生健康委员会重点医学科研项目(ZDB2020035)。

摘　　要：目的:研究甲状腺激素合成和代谢相关基因表达与内脏脂肪米色化的关系。方法:收集甲状腺功能正常的正常体重和肥胖者的内脏脂肪组织,荧光实时定量PCR检测甲状腺激素合成和代谢相关基因、内脏脂肪米色化功能蛋白和转录因子的mRNA表达,并进行相关性分析。结果:肥胖者内脏脂肪组织甲状腺激素转运体包括溶质载体家族16成员2和成员10(SLC16A2、SLC16A10)、溶质载体有机阴离子转运体家族成员1C1(SLCO1C1)、促甲状腺激素β亚基(TSHB)和脱碘酶2(iodothyronine deiodinase 2,DIO2)的mRNA表达均下降,其中SLC16A2、TSHB和DIO2差异有统计学意义。在校正年龄、性别、体重指数和FT3后内脏脂肪米色化功能蛋白UCP1及标志转录因子PR结构域家族成员16(PR domain-containing 16,PRDM16)的表达与上述基因表达呈正相关。结论:肥胖者内脏脂肪米色化功能下降与甲状腺激素合成和代谢密切相关。Objective:This study aims to study the relationship between thyroid hormone synthesis and metabolism-related gene expression and browning of visceral fat.Methods:The visceral fat tissues of normal weight and obese persons with normal thyroid function were collected,and the mRNA expression of genes related to thyroid hormone synthesis and metabolism,visceral fat beige functional protein and transcription factors were detected by fluorescence real-time quantitative PCR,then the correlation analysis was performed.Results:The mRNA expression of thyroid hormone transporters included solute carrier family 16 members 2 and 10(SLC16A2,SLC16A10),solute carrier organic anion transporter family member 1C1(SLCO1C1)thyroid stimulating hormoneβsubunit(TSHB)and iodothyronine deiodinase 2(DIO2)decreased significantly in the visceral adipose tissue of obese people.There were significant differences in SLC16A2,TSHB and DIO2 between two groups The expression of visceral fat beige functional protein UCP1 and marker transcription factor PR domain-containing 16(PR domain-containing 16,PRDM16)are positively correlated with the expression of the above genes after adjusted by age,gender,body mass indexand FT3.Conclusion:Decreased beige function of visceral fat in obese people is closely related to thyroid hormone synthesis and metabolism.

关 键 词：甲状腺素合成和代谢 内脏脂肪 脂肪米色化 肥胖 

分 类 号：R581[医药卫生—内分泌]