Validation of the Investigator 24plex QS Kit:a 6-dye multiplex PCR assay for forensic application in the Chinese Han population  

作　　者：Ruiyang Tao Chong Chen Xiang Sheng Ruocheng Xia Xiaochun Zhang Jingyi Zhang Zihao Yang Suhua Zhang Chengtao Li 

机构地区：[1]Institute of Forensic Medicine,West China School of Basic Medical Sciences&Forensic Medicine,Sichuan University,Chengdu,China [2]Shanghai Key Laboratory of Forensic Medicine,Shanghai Forensic Service Platform,Academy of Forensic Sciences,Ministry of Justice,Shanghai,China [3]College of Medicine and Forensics,Xi’an Jiaotong University Health Science Center,Xi’an,China [4]QIAGEN,Hilden,Germany [5]Department of Forensic Medicine,School of Basic Medical Science,Wenzhou Medical University,Wenzhou,China [6]Department of Forensic Science,Medical School of Soochow University,Suzhou,China

出　　处：《Forensic Sciences Research》2022年第2期172-180,共9页法庭科学研究（英文）

基　　金：This study was supported by the General Program of National Natural Science Foundation of China[grant number 81625013 and 81772028];the Shanghai Outstanding Academic Leaders Plan[grant number 2017485];the Shanghai Talent Development Funding[grant number 2017115].

摘　　要：The Investigator 24plex QS Kit(QIAGEN,Hilden,Germany)is a 6-dye fluorescent chemistry short tandem repeat(STR)polymerase chain reaction(PCR)amplification system that simultaneously amplifies 20 of the expanded Combined DNA Index System(CODIS)core STR loci,SE33,DYS391,and the standard sex-determining locus,amelogenin,as well as two special internal performance quality sensor controls(QS1 and QS2),which are included in the primer mix to check the PCR performance.This study was designed to be a pilot evaluation of this STR-PCR kit in a Chinese Han population regarding the PCR conditions,sensitivity,precision,accuracy,repeatability,reproducibility,and concordance;tolerance to PCR inhibitors;applicability to real“forensic-type”samples;species specificity;mixture,balance and stutter analyses,and utility in a population investigation.The exhaustive validation studies demonstrated that the Investigator 24plex QS system is accurate,sensitive and robust for STR genotyping.In addition,these genetic markers in the population data in our study indicated that they can also be useful for forensic identification and paternity testing in the Chinese Han population.

关 键 词：Forensic sciences forensic genetics short tandem repeat(STR)typing Investigator 24plex QS Kit validation study 

分 类 号：D919[医药卫生—法医学]