Determination of multiple drugs of abuse in human urine using dispersive liquid–liquid microextraction and capillary electrophoresis with PDA detection  

作　　者：Liang Meng Shuhai Ye Yilin Wu Linda You 

机构地区：[1]Department of Forensic Science,Fujian Police College,Fuzhou,China [2]Institute of Forensic Science,Fujian Provincial Public Security Department,Fuzhou,China

出　　处：《Forensic Sciences Research》2022年第2期265-271,共7页法庭科学研究（英文）

基　　金：The work was supported by the Programme of Outstanding Talents in the New Century in Universities of Fujian,China;Young Scientific Natural Science Foundation of Universities in Fujian.

摘　　要：A new method was developed for pre-concentration and determination of multiple drugs of abuse in human urine using dispersive liquid–liquid microextraction(DLLME)and capillary electrophoresis(CE)with photodiode array detection.The method was based on the formation of tiny droplets of an organic extractant in the prepared sample solution using water-immiscible organic solvent(chloroform)dissolved in water-miscible organic dispersive solvent(isopropyl alcohol).The organic phase,which extracted eight drugs of abuse from the prepared urine solution,was separated by centrifugation.The sedimented phase was transferred into a small volume CE auto-sampler vial with 10μL of 1%HCl methanol solution and evaporated to dryness.The residue was reconstituted in lidocaine hydrochloride(internal standard)aqueous solution and introduced by electrokinetic injection into CE.Under the optimum conditions,acceptable linear relationship was observed in the range of 3.0–500 ng/mL with the correlation coefficient(r)of 0.9982–0.9994 for spiked urine samples.The limit of detection(LOD)(S/N=3)was estimated to be 1.0 ng/mL.A recovery of 75.7%–90.6%was obtained for spiked samples.The mean relative error(MRE)was within±7.0%and the relative standard deviation(RSD)was less than 6.9%.The proposed DLLME-CE procedure offers an alternative analytical approach for the sensitive detection of drugs of abuse in real urine samples.

关 键 词：Forensic sciences forensic toxicology dispersive liquid-liquid microextraction capillary electrophoresis drugs of abuse urine sample 

分 类 号：D919[医药卫生—法医学]