脑络欣通及其拆方对右侧大脑中动脉闭塞再灌注大鼠学习记忆能力及突触可塑性的影响  

Effects of Naoluoxintong and its Disassembled Prescriptions on Learning and Memory Ability and Synaptic Plasticity in Right Middle Cerebral Artery Occlusion-reperfusion Rats

作　　者：秦雪梅 李佩佩[1] 时潇[1] 王丽娜[1] 贾根 胡建鹏[1] QIN Xuemei;LI Peipei;SHI Xiao;WANG Lina;JIA Gen;HU Jianpeng(School of Traditional Chinese Medicine,Anhui University of Chinese Medicine,Hefei,230038;Pizhou Hospital Affiliated to Xuzhou Medical University)

机构地区：[1]安徽中医药大学中医学院,安徽省合肥市230038 [2]徐州医科大学附属邳州医院

出　　处：《中医杂志》2022年第12期1163-1169,共7页Journal of Traditional Chinese Medicine

基　　金：国家中医药管理局全国中医学术流派传承工作室项目(国中医药人教函[2019]62号);安徽中医药大学第七届科技创新基金项目(2019ZD01)。

摘　　要：目的探讨脑络欣通防治缺血性脑卒中的可能作用机制。方法50只SD大鼠随机分为假手术组、模型组、脑络欣通组、益气组、活血通络组各10只。除假手术组外,其余各组大鼠采用改良线栓法制造大鼠右侧大脑中动脉缺血再灌注模型。造模成功后,脑络欣通组、益气组、活血通络组大鼠分别给予脑络欣通8.54 g/(kg·d)、益气方3.5 g/(kg·d)、活血通络方5.04 g/(kg·d)灌胃,假手术组和模型组大鼠均给予10 ml/(kg·d)的生理盐水灌胃,每日2次,共灌胃14天。采用Morris水迷宫(定位航行实验、空间探索实验)观察大鼠学习记忆能力,HE染色观察右侧额顶叶皮质及海马区病理学变化,免疫组化观察右侧额顶叶皮质和海马区谷氨酸受体1(GluR1)、钙/钙调素依赖性蛋白激酶Ⅱ(CaMKⅡ)表达变化。结果与假手术组比较,模型组大鼠各时间点逃避潜伏期均显著延长,在目标象限停留的时间及穿越平台的次数均减少,右侧额顶叶皮质及海马区GluR1、CaMKⅡ表达降低(P<0.01);与模型组比较,脑络欣通组、益气组、活血通络组大鼠各时间点逃避潜伏期均显著缩短(P<0.05),在目标象限停留的时间及穿越平台的次数明显增多(P<0.05),右侧额顶叶皮质及海马区GluR1、CaMKⅡ表达升高(P<0.01);与脑络欣通组比较,益气组、活血通络组大鼠各时间点逃避潜伏期延长,在目标象限停留的时间及穿越平台的次数减少,右侧额顶叶皮质及海马区GluR1、CaMKⅡ表达降低(P<0.05)。益气组和活血通络组各指标表达差异均无统计学意义(P>0.05)。组织病理学结果显示,与模型组相比,脑络欣通组、益气组、活血通络组神经细胞排列、形态大小均有所恢复,细胞水肿、坏死等现象改善。脑络欣通组比益气组、活血通络组改善病理损伤现象更明显。结论脑络欣通可改善右侧大脑中动脉闭塞再灌注大鼠学习记忆能力,全方效果优于各拆方,其作用机制�Objective To explore the possible mechanism of Naoluoxintong(脑络欣通)for prevention and treatment of ischemic stroke.Methods Naoluoxintong group,Yiqi(益气)group and Huoxue Tongluo(活血通络)group,with 10 rats in each group.Except for the sham operation group,the modified suture method was used in the other groups to establish the right middle cerebral ischemia-reperfusion model.After successful modeling,rats in the Naoluoxintong group,Yiqi group and Huoxue Tongluo group were given Naoluoxintong 8.54 g/(kg·d),Yiqi Formula 3.5 g(kg·d)and Huoxue Tongluo Formula 5.04 g/(kg·d)by gavage respectively,while the sham operation group and the model group were given the10 ml/(kg·d)of normal saline by gavage,twice a day,for a total of 14 days.Morris water maze(positioning navigation test,spatial navigation test)was used to observe the learning and memory ability of rats,while HE staining was used to observe the pathological changes in the right frontoparietal cortex and hippocampus,and immunohistochemistry was used to detect changes in the expression of glutamate receptor 1(GluR1)and calcium/calmodulin-dependent protein kinaseⅡ(CaMKⅡ)in the right frontoparietal cortex and hippocampus.Results those in the sham operation group,the escape latency of rats in the model group was significantly prolonged at each time point,while the time spent in the target quadrant and the times of crossing the platform decreased,and the expressions of GluR1 and CaMKⅡwere reduced(P<0.01).Compared with those in the model group,the escape latency of the rats in the Naoluoxintong group,the Yiqi group and the Huoxue Tongluo group at each time point was significantly shortened(P<0.05),while the time spent in the target quadrant and the times of crossing the platform increased significantly(P<0.05),and the expressions of GluR1 and CaMKII increased as well(P<0.01).Compared with those in the Naoluoxintong group,the escape latency in the Yiqi group and the Huoxue Tongluo group was prolonged at each time point,while the time spent in the

关 键 词：缺血性脑卒中 脑络欣通 学习记忆能力 突触可塑性 谷氨酸受体1 钙/钙调素依赖性蛋白激酶Ⅱ 

分 类 号：R285.5[医药卫生—中药学]