离子色谱法研究唾液葡萄糖与血糖水平的相关性  

Correlation between saliva glucose and blood glucose levels by ion chromatography

作　　者：窦倩 徐春[2] 戴庆[1] 朱泊羽 郭海健[3] 肖新华[4] Dou Qian;Xu Chun;Dai Qing;Zhu Boyu;Guo Haijian;Xiao Xinhua(CAS Key Laboratory of Nanophotonic Materials and Devices,CAS Center for Excellence in Nanoscience,National Center for Nanoscience and Technology,Beijing 100190,China;Department of Endocrinology,3rd Medical Center,PLA General Hospital,Beijing 100039,China;Jiangsu Provincial Center for Disease Control and Prevention,Nanjing 210009,China;Department of Endocrinology,Peking Union Medical College Hospital,Beijing 100730,China)

机构地区：[1]国家纳米科学中心,中国科学院卓越中心,中国科学院纳米光子材料与器件重点实验室,北京100190 [2]解放军总医院第三医学中心内分泌科,北京100039 [3]江苏省疾病预防控制中心,南京210009 [4]北京协和医院内分泌科,北京100730

出　　处：《中华内分泌外科杂志》2022年第3期269-273,共5页Chinese Journal of Endocrine Surgery

基　　金：中国科学院科技服务网络计划(STS计划)(KFJ-STS-ZDTP-063)。

摘　　要：目的采用高精度离子色谱仪测试受试者唾液葡萄糖(唾液糖)浓度,分析其与血糖水平的相关性,为唾液糖作为血糖监测的辅助指标提供临床数据。方法纳入糖代谢正常(NGT)者和糖尿病(DM)患者共计268例,同时采集受试者空腹静脉血和唾液样本。分别利用离子色谱仪、全自动生化分析仪和糖化血红蛋白分析仪测定受试者的唾液糖、血糖和糖化血红蛋白(HbA1c)水平。组间比较采用Kruskal-Wallis H秩和检验,相关性分析采用Spearman相关法。结果DM组受试者血糖、唾液糖和HbA1c平均值都高于NGT组,且差异均具有统计学意义(P<0.05)。唾液糖的切点分别设定为10、15、20、25 mg/L,当唾液糖浓度大于等于上述切点水平时,唾液糖与血糖水平呈正相关(相关系数r分别为0.321、0.379、0.509、0.428,P均<0.05)。结论DM患者的唾液糖水平明显高于NGT人群,当唾液糖浓度≥20 mg/L时,唾液糖与血糖水平呈显著正相关,且此时相关系数r最大。Objective To analyze the correlation between saliva glucose and blood glucose level by measuring the concentration of saliva with high-precision ion chromatograph,and further to provide the clinical data for saliva glucose as an auxiliary index of blood glucose monitoring.Methods A total of 268 subjects with normal glucose metabolism(NGT)and diabetes mellitus(DM)were enrolled and fasting venous blood and saliva samples were collected at the same time.The levels of saliva glucose,blood glucose and glycosylated hemoglobin(HbA1c)were measured by ion chromatograph,automatic biochemical analyzer and glycosylated hemoglobin analyzer,respectively.Methods of Kruskal-Wallis H test was used for comparison between groups,and the Spearman correlation was used for correlation analysis.Results The mean values of blood glucose,saliva glucose and HbA1c in the DM group are all higher than those in the NGT group,and the differences are all statistically significant(P<0.05).Saliva glucose cut-off points are set at 10,15,20 and 25 mg/L,respectively.When the saliva glucose concentration is greater than or equal to the above cut-off points,the saliva glucose level are positively correlated with the blood glucose level(r=0.321,0.379,0.509 and 0.428,respectively,P<0.05).Conclusion The level of saliva glucose in DM is significantly higher than that in NGT.When the concentration of saliva glucose is greater than 20 mg/L,there is a significant positive correlation between saliva glucose and blood glucose,and the max correlation coefficient r is 0.509.

关 键 词：糖尿病 无创血糖监测 唾液葡萄糖 离子色谱 

分 类 号：R587[医药卫生—内分泌]