miR-92a-3p在老年骨质疏松中表达及其在髋部脆性骨折中的诊断价值  

Dysregulated expression of miR-92a-3p in senile osteoporosis and its diagnostic value in hip fragility fractures

作　　者：贾彩虹[1] 闫宏[1] 杨书艳[1] Jia Caihong;Yan Hong;Yang Shuyan(Department of Laboratory,the Second Hospital of Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]山西医科大学第二医院检验科,太原030001

出　　处：《中华内分泌外科杂志》2022年第3期361-366,共6页Chinese Journal of Endocrine Surgery

基　　金：山西省卫生健康委课题计划(2020126)。

摘　　要：目的探讨miR-92a-3p在老年骨质疏松症(osteoporosis,OP)中的表达情况及其在髋部脆性骨折中的诊断价值。方法借助美国国家生物技术信息中心(National Center for Biotechnology Information,NCBI)网站检索与OP、miRNA相关的数据集并进行分析与筛选获得目标miRNA。收集2020年1月至2020年6月期间,从山西医科大学第二医院骨科纳入53例OP患者与24例健康人的血清标本,将OP患者分为发生髋部脆性骨折组(OP_F组,30例)和未发生髋部脆性骨折组(OP_WF组,23例),分析受试者的骨密度与血清中的骨代谢标志物测量:钙、磷、甲状旁腺激素、25-羟基维生素D、骨碱性磷酸酶、血清c末端肽。利用qRT-PCR检测血清标本中目标miRNA的表达水平。χ^(2)检验、t检验、斯皮尔曼等级相关、接收者操作特征曲线(receiver operating characteristic curve,ROC曲线)分析miR-92a-3p表达水平与患者临床特征的潜在关系。结果从NCBI网站中分析得出miR-92a-3p在OP患者中表达较健康人水平升高(t=3.41,P=0.007),miR-1304-5p表达水平降低(t=5.13,P<0.001)。qRT-PCR实验证实以上结果,并进一步发现较OP_WF组,OP_F组中miR-92a-3p表达明显升高(t=3.01,P=0.004),但miR-1304-5p在两组间无明显差异(t=0.71,P=0.480)。OP患者组与健康对照组相比,BMI(t=2.71,P=0.008)、骨密度评分(t=29.02,P<0.001)、钙(t=61.20,P<0.001)、磷(t=2.54,P=0.013)、25-羟基维生素D(t=3.01,P=0.004)、骨碱性磷酸酶(t=12.56,P<0.001)、血清c末端肽(t=7.52,P<0.001)水平差异有统计学意义。OP_F组与OP_WF组相比,骨密度评分(t=2.08,P=0.042)、钙(t=15.75,P<0.001)、骨碱性磷酸酶(t=2.02,P=0.049)、血清c末端肽(t=3.39,P=0.001)的水平差异有统计学意义。骨密度评分、骨碱性磷酸酶浓度与miR-92a-3p的表达有关且分别呈负相关(r=-0.403,P=0.027)与正相关(r=0.416,P=0.022)。ROC曲线下面积为0.723,miR-92a-3p水平对诊断髋部脆性骨折具有潜在意义(P=0.006)。结论miR-92a-3p在OP中高表达,�Objective To investigate the expression of miR-92a-3p in senile osteoporosis(OP)and its diagnostic value in hip fragility fractures.Methods With the help of the National Center for Biotechnology Information(NCBI)website,the data sets related to OP and miRNA were retrieved and analyzed and screened to obtain the target miRNA.Serum samples were collectedfrom 53 OP patients and 24 healthy people,and the OP patients were divided into hip fragility fracture group(OP_F;n=30)and no hip fragility fracture group(OP_WF;n=23).The subjects'bone mineral density and serum bone metabolism markers were measured:calcium,phosphorus,parathyroid hormone,25-hydroxyvitamin D,bone alkaline phosphatase,serum C-terminal peptide.The expression levels of target miRNAs in serum samples were detected by qRT-PCR.Chi-square test,t test,Spearman rank correlation and receiver operating characteristic curve(ROC curve)were used to analyze the potential relationship between the expression level of miR-92a-3p and the clinical characteristics of patients.Results According to the analysis of NCBI website,the expression of miR-92a-3p in OP patients was higher(t=3.41,P=0.007)than that in healthy people,and the expression level of miR-1304-5p was lower(t=5.13,P<0.001).qRT-PCR experiments confirmed the above results,and further found that the expression of miR-92a-3p in the OP_F group was significantly higher than that in the OP_WF group(t=3.01,P=0.004),but there was no significant difference in miR-1304-5p between the two groups(t=0.71,P=0.480).Compared with the healthy control group,the BMI(t=2.71,P=0.008),bone mineral density score(t=29.02,P<0.001),calcium(t=61.20,P<0.001),phosphorus(t=2.54,P=0.013),25-hydroxyvitamin D(t=3.01,P=0.004),bone alkaline phosphatase(t=12.56,P<0.001),and serum C-terminal peptide(t=7.52,P<0.001)levels were statistically different between the OP patient group and the healthy control group.Compared with the OP_WF group,the bone mineral density score(t=2.08,P=0.042),calcium(t=15.75,P<0.001),bone alkaline phosphatase(t=2.02,P=0.04

关 键 词：miR-92a-3p 骨质疏松症 髋部脆性骨折 骨密度 骨碱性磷酸酶 血清c末端肽 

分 类 号：R658.3[医药卫生—外科学] R738.1[医药卫生—临床医学]