LncRNA-ANCR在人脑胶质瘤组织及细胞中的表达及其与细胞恶性增殖的关系  

Mechanism of LncRNA-ANCR expression and cellular malignant proliferation in human glioma tissues and cells

作　　者：王海涛 苏赢 韩秀斌 孔祥铨 许鹏 Wang Haitao;Su Ying;Han Xiubin;Kong Xiangquan;Xu Peng(Department of Neurosurgery,Shandong Linyi Central Hospital,Linyi 276400,China)

机构地区：[1]山东省临沂市中心医院神经外科,临沂276400

出　　处：《中华内分泌外科杂志》2022年第3期367-371,共5页Chinese Journal of Endocrine Surgery

基　　金：山东省高等学校科研计划科技类项目(J16LL59)。

摘　　要：目的探究LncRNA ANCR在人脑胶质瘤组织中的表达及其与细胞恶性增殖间的关系。方法收集2017年1月5日至2018年9月30日临沂市中心医院神经外科45例高级别脑胶质瘤组织作为高级别组、13例低级别脑胶质瘤组织作为低级别组、10例正常脑外伤组织作为正常组,荧光定量PCR技术(qPCR)检测组织中ANCR及潜在的eIF4B的表达。体外采用慢病毒转染shRNA表达载体,在人高级别脑胶质瘤U251细胞中构建对照细胞及ANCR干扰细胞株,作为本研究的对照组、实验1组及实验2组细胞,qPCR检测细胞中ANCR、eIF4B及下游分子c-Myc mRNA的表达水平,蛋白印迹实验(Western blot)检测eIF4B及c-Myc蛋白表达水平,CCK-8实验检测细胞相对增殖能力变化,平板集落形成实验检测细胞克隆形成能力变化。使用SPSS 21.0进行统计分析,组间比较采用方差分析,组内比较使用SNK-q两两比较法。结果高级别脑胶质瘤、低级别脑胶质瘤及正常脑外伤组织中ANCR mRNA的表达水平分别为0.710±0.125、2.033±0.312及3.408±0.296,而eIF4B mRNA的表达水平为0.176±0.019、0.268±0.022及0.426±0.028,ANCR及eIF4B在高级别脑胶质瘤组织中表达高于低级别脑胶质瘤及正常脑组织(P<0.001),ANCR在低级别脑胶质瘤组织中表达高于正常脑组织(P=0.013)。ANCR与eIF4B mRNA两者差异具有统计学意义(P<0.001)。对照组、实验1组及实验2组ANCR mRNA的表达分别为1.000±0.021、0.202±0.057及0.300±0.016;对照组、实验1组及实验2组eIF4B mRNA的表达分别为1.000±0.078、0.452±0.012及0.526±0.037;对照组、实验1组及实验2组c-Myc mRNA的表达分别为1.000±0.053、0.688±0.067及0.564±0.089,实验1组及实验2组细胞中ANCR、eIF4B及c-Myc mRNA及蛋白分子表达显著低于对照组细胞(P<0.001);实验1组及实验2组细胞在72 h及96 h增殖能力显著降低,克隆形成能力显著减弱(P<0.001)。结论ANCR在高级别脑胶质瘤组织中表达显著上调,且与eIF4B表达正相�Objective To investigate the expression of LncRNA ANCR in human glioma tissues and its relationship with malignant proliferation of cells.Methods The samples of 10 normal brain tissue,13 low-grade and 45 high-grade gliomas were regarded as normal group,low-grade group and high-grade group,which were collected from neurosurgery department in Linyi Central Hospital,and the expression of ANCR and potential interaction molecule eIF4B was detected by reverse transcription polymerase chain reaction(RT-PCR)in vitro.Lentivirus transfection in vitro was used to construct the U251 shRNA ANCR and control cell line in human high-grade gliomas as control,test 1 and test 2 group cells in the study.QPCR detect the expression level ANCR,eIF4B and Myc mRNA in cells.Western blot was used to detect the expression of eIF4B and c-Myc protein,CCK-8 assay was used to detect the relative proliferation ability of cells,and the colony formation assay was used to observe the change of cell clone formation.SPSS 21.0 was used for statistical analysis,analysis of variance was used for inter group comparison,and SNK-q pairwise comparison method was used for intra group comparison.Results The expressions of ANCR mRNA in high-grade glioma tissues,low-grade gliomas and normal brain tissues were 0.710±0.125,2.033±0.312 and 3.408±0.296.The expressions of eIF4B mRNA in high-grade glioma tissues,low-grade gliomas and normal brain tissues were 0.176±0.019,0.268±0.022 and 0.426±0.028.The expression of ANCR and eIF4B in high-grade glioma tissues was higher than that in low-grade gliomas and normal brain tissues(P<0.001).The expression of ANCR in low-grade glioma tissues was higher than that in normal brain tissues(P=0.013).There was a significant positive correlation between the expression of ANCR and eIF4B in glioma tissues(P<0.001);The expressions of ANCR mRNA in Control,test1 and test2 were 1.000±0.021,0.202±0.057 and 0.300±0.016.The expressions of eIF4B mRNA were 1.000±0.078,0.452±0.012 and 0.526±0.037,and the expressions of c-Myc mRNA w

关 键 词：脑胶质瘤 肿瘤转移 LncRNA 转录因子eIF4B 

分 类 号：R651.1+1[医药卫生—外科学] R736[医药卫生—临床医学]