大花旋覆花素对体外Hep G2细胞增殖、凋亡和mTORC1信号通路的影响  

Effects of britanin on cell proliferation,apoptosis and mTORC1 signaling pathway of Hep G2 cells in vitro

作　　者：任洁雯 李宗怡 任嘉欣 赖鸣杰 Ren Jiewen;Li Zongyi;Ren Jiaxin(Department of Gastroenterology,Affiliated Dongguan Hospital,Guangzhou University of Traditional Chinese Medicine,Guangzhou 510000,Guangdong Province,China)

机构地区：[1]广州中医药大学附属东莞医院消化内科,广州市510000 [2]广州中医药大学附属东莞医院内十二科,广州市510000 [3]东莞市莞城医院药剂科 [4]东莞市中西医结合医院消化内科

出　　处：《实用肝脏病杂志》2022年第4期468-471,共4页Journal of Practical Hepatology

基　　金：广东省自然科学基金资助项目(编号:2020502)。

摘　　要：目的研究大花旋覆花素肿瘤细胞增殖、凋亡和哺乳动物雷帕霉素靶蛋白(mTORC1)信号通路的影响。方法取Hep G2细胞,分组给予0μmol/L、5μmol/L、10μmol/L和20μmol/L大花旋覆花素处理48 h,采用MTT法检测细胞增殖,使用流式细胞仪检测细胞凋亡,采用Western bloting法检测凋亡相关蛋白Bax、Bcl2、Caspase3及mTORC1信号通路蛋白mTORC1和70S6K表达。结果5μmol/L、10μmol/L和20μmol/L大花旋覆花素处理组细胞增殖率分别为(89.56±8.11)%、(66.40±6.61)%和(41.78±5.79)%,均显著低于0μmol/L大花旋覆花素对照组【(100.00±10.01)%,P<0.05】;细胞凋亡率分别为(14.75±1.34)%、(19.11±1.87)%和(27.45±1.99)%,均显著高于对照组【(7.01±0.89)%,P<0.05】;促凋亡蛋白Bax和Caspase3蛋白表达分别为(1.36±0.15)和(1.63±0.32)、(3.57±0.33)和(3.92±0.47)、(7.33±0.52)和(6.94±0.53),显著高于对照组【分别为(0.98±0.11)和(0.87±0.15),P<0.05】,而抑凋亡蛋白Bcl2表达分别为(4.71±0.52)、(2.36±0.36)和(0.89±0.14),显著低于对照组【(8.05±0.65),P<0.05】;mTORC1和70S6K蛋白表达分别为(0.82±0.08)和(0.79±0.08)、(0.63±0.06)和(0.58±0.05)、(0.51±0.05)和(0.43±0.04),显著低于对照组【(0.96±0.11)和(0.99±0.09),P<0.05】。结论大花旋覆花素可通过下调mTORC1信号通路抑制肝癌细胞体外增殖,诱导其凋亡,发挥显著的抗肿瘤效应。Objective The aim of this study was to explore the effects of britanin on cell proliferation and apoptosis and impact on mammalian target of rapamycin complex(mTORC1)signal pathway in HepG2 cells in vitro.Methods The Hep G2 cells were cultured with britanin at 0μmol/L(control),5μmol/L,10μmol/L and 20μmol/L for 48 hours.The cell proliferation was detected by MTT,the cell apoptosis was evaluated by flow cytometry,and the apoptosis-related protein expressions,such as Bax,Bcl2 and Caspase3 and mTORC1 signaling pathway were detected by Western bloting.Results The cell proliferation rates in 5μmol/L,10μmol/L and 20μmol/L britanin-intervened groups were(89.56±8.11)%,(66.40±6.61)%and(41.78±5.79)%,all significantly lower than[(100.00±10.01)%,P<0.05]in the control;the apoptosis rate were(14.75±1.34)%,(19.11±1.87)%and(27.45±1.99)%,significantly higher than[(7.01±0.89)%,P<0.05]in the control;the expressions of pro-apoptotic proteins,Bax and Caspase3,were(1.36±0.15)and(1.63±0.32),(3.57±0.33)and(3.92±0.47),and(7.33±0.52)and(6.94±0.53),all significantly higher than[(0.98±0.11)and(0.87±0.15),P<0.05]in the control,while the expression of anti-apoptotic protein,Bcl2,were(4.71±0.52),(2.36±0.36)and(0.89±0.14),significantly lower than[(8.05±0.65),P<0.05]in the control;the protein expressions of mTORC1 and 70S6K were(0.82±0.08)and(0.79±0.08),(0.63±0.06)and(0.58±0.05)and(0.51±0.05)and(0.43±0.04),all significantly lower than[(0.96±0.11)and(0.99±0.09),P<0.05]in the control.Conclusion Britanin could inhibit cell proliferation and induce cell apoptosis,which might exert a certain anti-tumor effects by down-regulation of mTORC1 signaling pathway.

关 键 词：Hep G2细胞 大花旋覆花素 细胞增殖 凋亡 mTORC1信号通路 

分 类 号：R285[医药卫生—中药学]