基于高通量测序的5种蜘蛛所携带的细菌多样性比较  

Comparative Bacterial Diversity Among Five Species of Spiders Based on High-throughput Sequencing

作　　者：高瑛 韩瑜 王玥琪 ALI Abid 崔淑艳 郑国 GAO Ying;HAN Yu;WANG Yue-qi;ALI Abid;CUI Shu-yan;ZHENG Guo(College of Life Sciences,Shenyang Normal University,Shenyang 110034,Liaoning,China;Department of Entomology,University of Agriculture,Faisalabad 38040,Pakistan)

机构地区：[1]沈阳师范大学生命科学学院,中国辽宁沈阳110034 [2]费萨拉巴德农业大学昆虫学系,巴基斯坦费萨拉巴德38040

出　　处：《生命科学研究》2022年第3期229-235,共7页Life Science Research

基　　金：国家自然科学基金面上项目(31970410);辽宁省兴辽英才计划项目(XLYC2002083);辽宁省重点研发计划项目(2018103004)。

摘　　要：为了解不同蜘蛛所携带的细菌多样性及群落组成的差异,基于16S r RNA基因测序技术,对采自辽宁省沈阳市的5种常见蜘蛛所携带的细菌群落进行了比较。结果表明:5种蜘蛛共注释到34门421科948属细菌。变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes)是5种蜘蛛共有的核心菌门(>1%),土壤杆菌属(Sediminibacterium)、苍白杆菌属(Ochrobactrum)、无氧芽孢杆菌属(Anoxybacillus)、贪铜菌属(Cupriavidus)和溶杆菌属(Lysobacter)是共有的核心菌属(>1%)。多样性分析显示,地表游猎的星豹蛛细菌群落的各α多样性指数均最高。PCA分析结果表明,环境因素对蜘蛛所携带的细菌群落有重要影响。研究结果对进一步了解蜘蛛所携带细菌的多样性、群落结构及其影响因素有参考价值。The focus of this study was to understand the diversity and composition of bacteria among different species of spiders.Five species of spiders were collected from community park in Shenyang City,Liaoning Province,and composition and diversity of the associated bacteria were compared based on 16S rRNA gene sequencing technology.The results showed that a total of 34 phyla,421 families and 948 genera of associated bacteria were identified from the spider hosts.At the phylum level,Proteobacteria,Bacteroidetes and Firmicutes were the dominant bacteria(>1%)in all the five spider species.At the genus level,Sediminibacterium,Ochrobactrum,Anoxybacillus,Cupriavidus and Lysobacter were the dominant bacteria(>1%).Moreover,alpha diversity analysis showed that all diversity indices of associated bacteria from Pardosa astrigera were the highest.The results of PCA demonstrated that environmental factors should play a significant role in the composition and diversity of spider-associated bacteria.The results provide a reference for understanding the spider-associated bacterial diversity,community structure and influencing factors in further research.

关 键 词：蜘蛛目 16S rRNA测序 线性判别分析效应量(LEf Se) 生物多样性 

分 类 号：Q938.[生物学—微生物学]