基于微卫星分子标记的浙江省不同滞育型迷卡斗蟋的遗传分化分析  

Analysis of the genetic differentiation of Velarifictorus micado(Orthoptera:Gryllidae)with different diapause types in Zhejiang Province of eastern China based on microsatellite markers

作　　者：王佰秋 沈初泽 武雪 谢慧聪 宗靖淞 余哲媛 白芸 李恺[1] 何祝清[1] WANG Bai-Qiu;SHEN Chu-Ze;WU Xue;XIE Hui-Cong;ZONG Jing-Song;YU Zhe-Yuan;BAI Yun;LI Kai;HE Zhu-Qing(School of Life Sciences, East China Normal University, Shanghai 200241, China;College of Life Sciences, Beijing Normal University, Beijing 100875, China)

机构地区：[1]华东师范大学生命科学学院,上海200241 [2]北京师范大学生命科学学院,北京100875

出　　处：《昆虫学报》2022年第6期782-790,共9页Acta Entomologica Sinica

基　　金：国家自然科学基金青年项目(31801997);上海市自然科学基金项目(19ZR1416100)。

摘　　要：【目的】迷卡斗蟋Velarifictorus micado存在两种显著不同的滞育型,即卵滞育和若虫滞育,而在长江流域附近两种滞育型种群存在广泛同域分布。本研究旨在探究浙江省同域分布不同滞育型迷卡斗蟋种群之间的遗传分化的情况。【方法】以浙江省百山祖、大盘山和天童山3个地区的不同滞育型的迷卡斗蟋6个种群共134个个体为研究材料,筛选具较高多态性的微卫星位点,并使用筛选获得的微卫星位点对这6个种群共134个个体进行种群遗传学分析。【结果】筛选出11对具较高多态性的微卫星位点;全部种群的平均等位基因数为13.803,平均观测杂合度为0.743,其中百山祖卵滞育种群观测杂合度最低,与其他种群间遗传分化最大。百山祖卵滞育种群与其他种群之间的基因交流程度很低,其余种群间均显示出高水平的基因流。【结论】浙江迷卡斗蟋不同地理种群及不同滞育型特征种群间遗传分化程度均较低,存在较频繁的基因交流,尚未表现出物种分化的趋势。【Aim】Velarifictorus micado has two types of diapause,egg diapause and nymphal diapause,which are sympatrically distributed in the Yangtze River basin.The objective of this study is to explore the genetic differentiation of V.micado populations between different diapause types sympatrically distributed in Zhejiang Province of eastern China.【Methods】Microsatellite loci with high polymorphism were screened with materials of 134 individuals of six populations of V.micado with different diapause types from three areas,Baishanzu,Dapanshan and Tiantongshan,in Zhejiang Province,and the population genetic analysis of the 134 individuals of six populations of V.micado was performed using the screened microsatellite loci.【Results】Eleven microsatellite loci with high polymorphism were obtained.For all populations,the mean number of alleles was 13.803,and the mean observed heterozygosity was 0.743.The observed heterozygosity of egg diapause populations from Baishanzu was the lowest,indicating the largest genetic differentiation with other populations.Besides,the gene flow between egg diapause populations from Baishanzu and the other populations was low,while the other populations showed higher levels of gene flow.【Conclusion】There is low degree of genetic differentiation and high level of gene flow among different populations of V.micado with different diapause types in Zhejiang,showing no trend of species differentiation.

关 键 词：迷卡斗蟋 滞育型 微卫星 种群遗传学 基因流 遗传多样性 

分 类 号：Q968[生物学—昆虫学]