病毒学抑制的HIV-1感染者免疫衰老相关CD4^(+)T淋巴细胞亚群的分析  

Analysis of CD4^(+)T cell subsets of immunosenescence in virologically suppressed HIV-1 infected persons

作　　者：郭悦 张燕琳[1] 朱丹 龚芳红[1] 高钰双 朱昆蓉[1] 李姗珊 GUO Yue;ZHANG Yan-lin;ZHU Dan;GONG Fang-hong;GAO Yu-shuang;ZHU Kun-rong;LI Shan-shan(Chengdu Center for Disease Control and Prevention,Chengdu,Sichuan 610041,China)

机构地区：[1]成都市疾病预防控制中心性病艾滋病检测科,四川成都610041

出　　处：《现代预防医学》2022年第12期2262-2270,共9页Modern Preventive Medicine

摘　　要：目的了解病毒学抑制的HIV-1感染者CD4^(+)T淋巴细胞免疫衰老状态,探索主成分分析法能否用于评价HIV-1患者的免疫衰老。方法选择抗逆转录疗法治疗半年以上成年病毒学抑制HIV-1感染者,根据CD4^(+)T淋巴细胞计数结果分为CD4无缺陷组(≥500个/μl)和缺陷组(<500个/μl),每组65人,另选择22名未暴露且HIV-1抗体检测阴性者作为对照组,采用流式细胞仪检测其CD4^(+)T淋巴细胞(CD45RA;CD27;)、活化CD4^(+)T淋巴细胞(HLADR;CD38;)和复制性衰老CD4^(+)T淋巴细胞(CD57;CD28-)水平,运用主成分分析对三种细胞进行综合分析。结果缺陷组(27.64%)和无缺陷组(32.04%)的初始CD4^(+)T淋巴细胞较对照组(51.27%)有明显下降(F=24.35,P<0.001),活化CD4^(+)T淋巴细胞(14.26%,13.03%)较对照组(2.35%)有明显上升(F=19.75,P<0.001);缺陷组(12.64%)的复制性衰老CD4^(+)T淋巴细胞较无缺陷组(7.36%)和对照组(3.58%)有明显升高(F=6.68,P=0.002),而无缺陷组和对照组之间无统计学差异;主成分分析能区分出三组对象CD4^(+)T淋巴细胞免疫衰老的差异程度:缺陷组>无缺陷组>对照组(F=20.787,P<0.001)。结论病毒学抑制良好的HIV-1感染者即使CD4^(+)T细胞数量正常也表现为免疫衰老,CD4^(+)T细胞数量异常的人免疫衰老状态更严重,可运用主成分分析对病毒学抑制的HIV-1感染者免疫衰老相关细胞进行综合分析。Objective To understand the differences in the expression of immunosenescence-related cells in virologically suppressed HIV-1 infected persons,and to explore whether the comprehensive evaluation method can be used to evaluate the immunosenescence of HIV-1 patient.Methods Adult virologically suppressed HIV-1-infected patients treated with antiretroviral therapy for more than six months were selected and divided into CD4-free(≥500/μL)and defective(<500/μL)groups based on CD4^(+)T lymphocyte count results,with 65 individuals in each group,and 22 unexposed and HIV-1 antibody-test-negative individuals were selected as the control group,and their CD4^(+)T lymphocytes(CD45RA;CD27;),activated CD4^(+)T lymphocytes(HLA-DR;CD38;)and replicative senescent CD4^(+)T lymphocytes(CD57;CD28-)levels by flow cytometry,and the three cell types were analyzed together using principal component analysis.Results There was a significant decrease in initial CD4^(+)T lymphocytes(F=24.35,P<0.001)and a significant increase in activated CD4^(+)T lymphocytes(14.26%,13.03%)in the defective(27.64%)and nondefective groups(32.04%)compared to the control group(51.27%)(F=19.75,P<0.001);replicative senescent CD4^(+)T lymphocytes were significantly higher(F=6.68,P=0.002)in the defective group(12.64%)compared to the nondefective group(7.36%)and the control group(3.58%),while there was no statistical difference between the nondefective and control groups;principal component analysis was able to distinguish the degree of difference in im-munosenescence of CD4^(+)T lymphocytes among the three groups of subjects:defective group>non-defective group>control group(F=20.787,P<0.001).Conclusion HIV-1-infected individuals with good virological suppression exhibit immune senescence even with normal CD4^(+)T cell counts,and those with abnormal CD4^(+)T cell counts have a more severe state of immune senescence,and a comprehensive analysis of cells associated with immune senescence in virologically suppressed HIV-1-in-fected individuals can be performed using pr

关 键 词：人类免疫缺陷病毒 病毒学抑制 CD4^(+)T淋巴细胞 免疫衰老 主成分分析 

分 类 号：R512.91[医药卫生—内科学]