白及组培苗高效繁育技术研究  

Study on Efficient Propagation Technology of Bletilla striata Plantlets

作　　者：吴巧芬 夏科[1] 赵志国[1] 马晓雅 仇硕[1,2] WU Qiaofen;XIA Ke;ZHAO Zhiguo;MA Xiaoya;QIU Shuo(Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization,Guangxi Institute of Botany,Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences,Guilin,Guangxi,541006,China;College of Tourism&Landscape Architecture,Guilin University of Technology,Guilin,Guangxi,541006,China)

机构地区：[1]广西壮族自治区中国科学院广西植物研究所,广西植物功能物质研究与利用重点实验室,广西桂林541006 [2]桂林理工大学旅游与风景园林学院,广西桂林541006

出　　处：《广西科学院学报》2022年第2期181-187,共7页Journal of Guangxi Academy of Sciences

基　　金：广西重点研发计划项目(桂科AB18294026);广西科学院园艺植物种质创新及利用创新研究团队启动经费项目(CQZ-E-1919)资助。

摘　　要：为建立白及(Bletilla striata)组培苗高效繁育技术体系,以白及蒴果为外植体,通过设置不同培养基及不同移栽基质对白及种子无菌萌发、原球茎及丛生芽诱导、丛生芽生根及结鳞茎培养、移栽驯化以及驯化苗一年两季培育开展研究。结果表明:白及种子萌发、原球茎及丛生芽诱导最适培养基为1/2 MS+6苄基腺嘌呤(6 BA)1.0 mg/L+萘乙酸(NAA)0.1 mg/L+油菜素内酯(BR)0.01 mg/L+蔗糖20 g/L液体培养基;丛生芽生根及结鳞茎培养最适培养基为MS+6 BA 1.0 mg/L+BR 0.02 mg/L+NAA 0.2 mg/L+激动素(KT)0.5 mg/L+香蕉100 g/L+蔗糖30 g/L+琼脂5.0 g/L+活性炭1.0 g/L。丛生苗于3-4月移栽,最适宜的移栽基质为泥炭土、珍珠岩和蛭石(2∶2∶1,V∶V∶V)的组合物,移栽成活率为96.33%。一年两季的栽培管理并结合喷施0.02 mg/L BR能明显提高种苗质量。In order to establish an efficient propagation technology system of B.striata plantlets,the capsule of B.striata was used as the explants to study the aseptic germination of B.striata seeds,the induction of protocorm and cluster buds,the rooting of cluster buds,culture,domestication and transplantation of bulblets,and the cultivation of domestication seedlings in one year and two seasons by setting different media and different transplanting substrates.The results showed that the best culture medium for seed germination,protocorm and cluster buds induction of B.striata was liquid medium with 1/2 MS+6 BA 1.0 mg/L+NAA 0.1 mg/L+BR 0.01 mg/L+sucrose 20 g/L.The optimal medium for rooting bulblet culture was MS+6 BA 1.0 mg/L+BR 0.02 mg/L+NAA 0.2 mg/L+KT 0.5 mg/L+banana 100 g/L+sucrose 30 g/L+agar 5.0 g/L+activated carbon 1.0 g/L.Cluster seedlings were transplanted from March to April.The most suitable transplanting substrate was the composition of peat soil,perlite and vermiculite(2∶2∶1,V∶V∶V),and the transplanting survival rate was 96.33%.The the quality of B.striata seedlings can significantly improve treated with double cropping cultivation mode and spraying with 0.02 mg/L BR.

关 键 词：白及 无菌播种 组培快繁 移栽驯化 一年两季栽培 

分 类 号：S682.31[农业科学—观赏园艺]