lncRNA LINC00528通过调控Nrf2/HO-1信号通路对高糖诱导心肌细胞凋亡的机制研究  

The effects of long non-coding RNA-LINC00528 on high glucose-induced cardiomyocyte apoptosis by regulating the Nrf2/HO-1 signaling pathway

作　　者：江帆[1] 刘宁[2] 翟鑫[1] 管频[1] 董霄 JIANG Fan;LIU Ning;ZHAI Xin(Geriatric Medical Center,Hainan Provincial People’s Hospital,Hainan,Haikou 570311,China;不详)

机构地区：[1]海南省人民医院(海南医学院附属海南医院)老年医学中心,海口市570311 [2]海南省人民医院(海南医学院附属海南医院)胃肠外科,海口市570311

出　　处：《河北医药》2022年第13期1939-1942,1947,共5页Hebei Medical Journal

基　　金：海南省卫生健康行业科研项目(编号:20A200430);海南省自然科学基金青年基金项目(编号:819QN353)。

摘　　要：目的 探讨长链非编码RNA(lncRNA)LINC00528通过调控Nrf2/HO-1信号通路对高糖(high glucose,HG)诱导心肌细胞凋亡的机制研究。方法 通过体外培养H9C2心肌细胞建立细胞损伤模型,将细胞分组,分别为:Con组、HG组、HG+si-NC组、HG+si-LINC00528组、HG+si-LINC0052+pcDNA组和HG+si-LINC00528+Nrf2组。采用qRT-PCR检测LINC00528的表达;MTT检测细胞活力;流式细胞术检测细胞凋亡;Western blot检测Bcl-2、Bax和Nrf2/HO-1信号通路相关蛋白的表达;ELISA检测MDA含量和SOD活性。结果 HG组LINC00528表达、细胞凋亡率、Bax蛋白表达、MDA含量明显高于Con组(P<0.05);细胞活力、Bcl-2蛋白表达、SOD活性、Nrf2、HO-1、NQO1蛋白表达明显低于Con组(P<0.05)。HG+si-LINC00528组LINC00528表达、细胞凋亡率、Bax蛋白表达、MDA含量明显低于HG+si-NC组(P<0.05);细胞活力、Bcl-2蛋白表达、SOD活性、Nrf2、HO-1、NQO1蛋白表达明显高于HG+si-NC组(P<0.05)。HG+si-LINC00528+Nrf2组Nrf2蛋白、细胞活力、Bcl-2蛋白表达、SOD活性明显高于HG+si-LINC00528+pcDNA组(P<0.05);细胞凋亡率、Bax蛋白表达、MDA含量明显低于HG+si-LINC00528+pcDNA组(P<0.05)。结论 LINC00528通过上调Nrf2/HO-1信号通路减缓高糖诱导心肌细胞凋亡和氧化应激损伤。Objective To investigate the effects of long non-coding RNA(lncRNA)LINC00528 on high glucose(HG)-induced cardiomyocyte apoptosis by regulating the Nrf2/HO-1 signaling pathway,and to explre its action mechanism.Methods The cell injury models were established by culturing H9C2 cardiomyocytes in vitro,which were divided into six groups:control group,HG group,HG+si-NC group,HG+si-LINC00528 group,HG+si-LINC0052+pcDNA group and HG+si-LINC00528+Nrf2 group.The qRT-PCR was used to detect the expression of LINC00528;MTT detected cell viability;flow cytometry detected cell apoptosis;Western Blot detected the expression of Bcl-2,Bax and Nrf2/HO-1 signaling pathway related proteins;ELISA detected MDA content and SOD activity.Results The expression levels of LINC00528,Bax protein and MDA content as well as cell apoptosis rate in HG group were significantly higher than those in control group(P<0.05),however,the cell viability,SOD activity,and the expression levels of Bcl-2 protein,Nrf2,HO-1,NQO1Bcl-2 protein were significantly lower than those in control group(P<0.05),And the expression levels of LINC00528,Bax protein and MDA content as well as cell apoptosis rate in HG+si-LINC00528 group were significantly lower than those in HG+si-NC(P<0.05),however,the cell viability,SOD activity,and the expression levels of Bcl-2 protein,Nrf2,HO-1,NQO1 were significantly higher than those in HG+si-NC group(P<0.05),Moreover the expression levels of Nrf2 protein,Bcl-2 protein,and cell viability,SOD activity in HG+si-LINC00528+Nrf2 group were significantly higher than those in HG+si-LINC00528+pcDNAgroup(P<0.05),but,the cell,apoptosis rate,Bax protein expression,and MDA content were significantly lower than those in HG+si-LINC00528+pcDNAgroup(P<0.05).Conclusion LINC00528 can relieve the cardiomyocyte apoptosis and oxidative stress damage induced by high glucose through up-regulating the Nrf2/HO-1 signaling pathway.

关 键 词：LINC00528 Nrf2/HO-1信号通路 高糖 心肌细胞 凋亡 氧化应激 

分 类 号：R587.1[医药卫生—内分泌]