线粒体DNA T16189C变异与妊娠糖尿病的关系  

Relationship of mtDNA T16189C mutation and gestational diabetes mellitus

作　　者：张小勤 周玉兰[2] 付竹梅[3] 韩学学 展洁 ZHANG Xiaoqin;ZHOU Yulan;FU Zhumei;HAN Xuexue;ZHAN Jie(Department of Clinical Laboratory,Weifang Maternal and Child Health Hospital,Weifang 261011,Shandong,China;Department of Ultrasound,Weifang Maternal and Child Health Hospital,Weifang 261011,Shandong,China;Department of Obstetrics,Weifang Maternal and Child Health Hospital,Weifang 261011,Shandong,China)

机构地区：[1]潍坊市妇幼保健院检验科,山东潍坊261011 [2]潍坊市妇幼保健院超声科,山东潍坊261011 [3]潍坊市妇幼保健院产科,山东潍坊261011

出　　处：《检验医学》2022年第5期413-416,共4页Laboratory Medicine

基　　金：潍坊市科技发展计划项目(2021YX053)。

摘　　要：目的探讨线粒体DNA(mtDNA)T16189C变异与妊娠糖尿病(GDM)发生风险的关系。方法选取GDM孕妇204例(GDM组),以正常孕妇220名作为对照组。收集所有孕妇的一般资料[年龄、孕周、体质量指数(BMI)]和生化指标[空腹血糖(FBG)、口服葡萄糖耐量试验(OGTT)1 h血糖、OGTT 2 h血糖、糖化血红蛋白(HbA_(1c))、空腹胰岛素(FINS)]的检测结果,计算胰岛素抵抗指数(HOMAIR)。采用测序法检测mtDNA 16189位点的变异情况。结果GDM组mtDNA T16189C变异频率高于对照组(P<0.05)。携带mtDNA T16189C变异位点是GDM发生的危险因素(OR=1.36,95%CI为1.020~1.823)。根据是否携带mtDNA T16189C变异将GDM患者分为携带组和非携带组。携带组OGTT 1 h血糖、OGTT 2 h血糖、FINS、HOMA-IR水平高于非携带组(P<0.05);FBG、HbA_(1c) 2个组之间差异均无统计学意义(P>0.05)。结论GDM孕妇mtDNA T16189C变异频率显著升高。mtDNA T16189C是GDM发生的风险基因位点。Objective To study the relationship between the mutation of mitochondrial DNA(mtDNA)T16189C and the risk of gestational diabetes mellitus(GDM).Methods Totally,204 patients with GDM were enrolled as GDM group,and 220 healthy pregnant women were enrolled as control group.The general data of all the pregnant women,including age,gestational week,body mass index(BMI),fasting blood glucose(FBG),oral glucose tolerance test(OGTT)1 h blood glucose,OGTT 2 h blood glucose,glycated hemoglobin A_(1c)(HbA_(1c))and fasting insulin(FINS),were collected.The homeostasis model assessment for insulin resistance(HOMA-IR)was calculated.The genotype of mtDNA 16189 mutation was determined by sequencing.Results The mutation rate of this site of GDM group was higher than that of control group(P<0.05).The mtDNA T16189C mutation can increase the risk of GDM[odds ratio(OR)=1.36,95%confidence interval(CI)1.020-1.823].GDM patients were classified into carrying group and non-carrying group according to the mtDNA T16189C mutation.The levels of OGTT 1 h blood glucose,OGTT 2 h blood glucose,FINS and HOMA-IR in carrying group were higher than those in non-carrying group(P<0.05).There was no statistical significance for FBG and HbA_(1c) between the 2 groups(P>0.05).Conclusions The mtDNA T16189C mutation can increase the risk of GDM,and mtDNA T16189C mutation is risk to GDM.

关 键 词：线粒体DNA 变异位点 妊娠糖尿病 

分 类 号：R446.7[医药卫生—诊断学]