基于网络药理学探讨青蒿改善心肾毒性的潜在作用机制  

Potential Mechanism of Artemisia annua Ameliorating Cardiotoxicity and Nephrotoxicity Based on Network Pharmacology

作　　者：廖奕娇 邹凌 何日明[1] 易铁钢[1] LIAO Yijiao;ZOU Ling;HE Riming;YI Tiegang(The Fourth Clinical Medical School of Guangzhou University of Chinese Medicine,Shenzhen 518033,China;Huizhou Hospital of Guangzhou University of Chinese Medicine,Huizhou 516001,China)

机构地区：[1]广州中医药大学第四临床医学院,广东深圳518033 [2]广州中医药大学惠州医院,广东惠州516001

出　　处：《中医药信息》2022年第7期18-26,共9页Information on Traditional Chinese Medicine

基　　金：广东省自然科学基金-面上项目(2020A1515011151);深圳市科技计划项目(JCYJ20180302173708520);深圳市卫生计生系统科研项目(SZLY2018005)。

摘　　要：目的:基于网络药理学方法分析青蒿改善心肾毒性的作用机制,为实验研究、临床用药、新药靶点研发提供参考。方法:利用TCMSP、GeneCards数据库分别筛选获取中药青蒿的主要活性成分及其靶点以及心肾毒性相关疾病的主要靶点,由STRING平台对交集靶点进行PPI绘制分析。通过DAVID数据库对药物疾病交集靶点进行GO、KEGG富集,再采用Cytoscape 3.8.2软件构建“青蒿活性成分-靶点”和“青蒿活性成分-心肾毒性靶点-通路”网络图。结果:青蒿改善心肾毒性的主要活性成分是槲皮素、山柰酚、木犀草素和青蒿素,36个靶点中MAPK1、PTGS2、MAPK14、TP53、CASP3、TNF、MAPK8、CASP9、BCL2和BAX为重要靶标。获得GO富集条目共284个和KEGG富集通路82条,主要潜在通路为癌症相关通路、MAPK信号通路、凋亡相关通路、TNF信号通路、HIP-1信号通路和VEGF信号通路等。结论:青蒿中的多种成分可以通过调节MAPK、凋亡和炎症反应等相关的多分子、多通路以减轻心肾毒性。Objective:To analyze the action mechanism of Artemisia annua in ameliorating cardiotoxicity and nephrotoxicity based on network pharmacology,thus to provide reference for experimental research,clinical medication and new drug target discovery.Methods:TCMSP database and GeneCards database were used to screen the main active components,the targets of Artemisia annua,and the core targets of cardiotoxicity and nephrotoxicity related diseases.The PPI of the intersecting targets was mapped and analyzed by STRING database.Go and KEGG enrichment of intersection targets of drugs and diseases were conducted on DAVID database,and the network of'Artemisia annua active ingredients-targets'and'Artemisia annua active ingredients-cardiotoxicity and nephrotoxicity targets-pathways'were constructed by Cytoscape 3.8.2 software.Results:The main active components of Artemisia annua in alleviating cardiotoxicity and nephrotoxicity were Quercetin,Kaempferol,Luteolin and Artemisinin,among the 36 targets,MAPK1,PTGS2,MAPK14,TP53,CASP3,TNF,MAPK8,CASP9,BCL2 and Bax were the core targets.A total of 284 GO enrichment items and 82 KEGG enrichment pathways were obtained,and the main potential pathways were cancer-related pathways,MAPK signaling pathways,apoptosis-related pathways,TNF signaling pathway,HIP-1 signaling pathway and VEGF signaling pathway.Conclusion:The results indicates that several components of Artemisia annua can reduce cardiotoxicity and nephrotoxicity by regulating multiple molecules and pathways associated with MAPK,apoptosis and inflammation.

关 键 词：青蒿 恶性肿瘤 心肾毒性 网络药理 靶点预测 

分 类 号：R285[医药卫生—中药学]