短葶山麦冬茎尖愈伤组织的诱导与植株再生  

Callus Induction and Plant Regeneration by Shoot Tip of Liriope muscari(Decn.)Bailey

作　　者：袁玉虹[1] 董雯[2] 孙艳[2] 何天友[2] 郑郁善[2] Yuan Yuhong;Dong Wen;SunYan;He Tianyou;Zheng Yushan(Fujian Forestry Vocational&Technical College,Nanping,Fujian 353000,China;Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建林业职业技术学院,福建南平353000 [2]福建农林大学,福建福州350002

出　　处：《绿色科技》2022年第11期1-4,51,共5页Journal of Green Science and Technology

基　　金：国家科技支撑计划项目(编号:2009BAI73B01);2021年度福建省中青年教师教育科研项目(编号:JAT210756)。

摘　　要：以短葶山麦冬茎尖为外植体,进行了优化愈伤组织诱导与植物再生的最适激素配比试验。结果表明:诱导愈伤组织的最佳培养基为MS+2.0 mg/L 2,4-D+1.5 mg/L 6-BA+1.0 mg/LNAA,愈伤组织紧密块状,生长状况良好,诱导率达94.55%;愈伤组织继代培养最佳培养基为MS+1.0 mg/L 2,4-D+1.50 mg/L 6-BA+0.5 mg/LNAA,愈伤组织净增鲜重为1.08g,增殖系数达到5.1;愈伤组织分化不定芽最佳培养基在MS+4 mg/L6-BA+0.5 mg/LNAA,不定芽增殖系数达到5.54,最有利于不定芽形成。Plantlet shoot tips in vitro of Liriope muscari(Decn.)Bailey were usedas the explants to optimize the optimal hormone ratio for callus induction and plant regeneration.The results showed that the best medium for inducing callus was MS+2.0 mg·L-12,4-D+1.5 mg·L-16-BA+1.0 mg·L-1NAA,and the callus was dense and massive.The growth condition was good,and the induction rate was 94.55%;the optimal medium for subculture of callus is MS+1.0 mg·L-12,4-D+1.50 mg·L-16-BA+0.5 mg·L-1NAA,and the net fresh weight of callus is 1.08g.The proliferation coefficient reaches 5.1;the optimal medium for callus differentiation of adventitious buds was MS+4 mg·L-16-BA+0.5 mg·L-1NAA,and the proliferation coefficient of adventitious buds reached 5.54,which was most favorable for the formation of adventitious buds.

关 键 词：短葶山麦冬 组织培养 愈伤组织 继代 不定芽 

分 类 号：S567[农业科学—中草药栽培]