Determination of growth kinetics of microorganisms linked with 1,4-dioxane degradation in a consortium based on two improved methods  

作　　者：Yi Xiong Boya Wang Chao Zhou Huan Chen Gang Chen Youneng Tang 

机构地区：[1]Department of Civil and Environmental Engineering,FAMU-FSU College of Engineering,Florida State University,Tallahassee,FL 32310,USA [2]Geosyntec Consultants Inc.,Huntington Beach,CA 92648,USA [3]National High Magnetic Field Laboratory,Tallahassee,FL 32310,USA

出　　处：《Frontiers of Environmental Science & Engineering》2022年第5期97-107,共11页环境科学与工程前沿（英文）

基　　金：supported by Geosyntec Consultants through contract RF02700.

摘　　要：The conventional method for determining growth kinetics of microbial consortia relies on the total biomass concentration.This may be inaccurate for substrates that are uncommon in nature and can only be degraded by a small portion of the microbial community.1,4-dioxane,an emerging contaminant,is an example of such substrates.In this work,we evaluated an improved method for determining the growth kinetics of a 1,4-dioxane-degrading microbial consortium.In the improved method,we considered only bacterial taxa whose concentration increase correlated to 1,4-dioxane concentration decrease in duplicate microcosm tests.Using PEST(Parameter Estimation),a modelindependent parameter estimator,the kinetic constants were estimated by fitting the Monod kineticsbased simulation results to the experimental data that consisted of the concentrations of 1,4-dioxane and the considered bacterial taxa.The estimated kinetic constants were evaluated by comparing the simulation results with experimental results from another set of microcosm tests.The evaluation was quantified by the sum of squared relative residual,which was four orders of magnitude lower for the improved method than the conventional method.By further dividing the considered bacterial taxa into oligotrophs and copiotrophs,the sum of squared relative residual further decreased.

关 键 词：BIODEGRADATION 1 4-Dioxane KINETICS Microbial consortium 16S rRNA 

分 类 号：Q93[生物学—微生物学]