沈阳地区一起人副流感病毒3型引起的急性呼吸道感染暴发病原分子流行病学研究  

The molecularly etiological study on the human parainfluenza viruses caused an acute respiratory infection outbreak in Shenyang

作　　者：王冰[1] 付敏 陈叶[1] 白丽娜[1] 齐英[1] 李天宝 王馨梦 梁佳元[3] Wang Bing;Fu Min;Chen Ye;Bai Lina;Qi Ying;Li Tianbao;Wang Xinmeng;Liang Jiayuan(Shenyang Center for Disease Control and Prevention,Shenyang 110031,China;Shenhe District Center for Disease Control and Prevention,Shenyang 110000,China;Liaoning Center for Disease Control and Prevention,Shenyang 110005,China)

机构地区：[1]沈阳市疾病预防控制中心,沈阳110031 [2]沈河区疾病预防控制中心,沈阳11001 [3]辽宁省疾病预防控制中心,沈阳110005

出　　处：《中华实验和临床病毒学杂志》2022年第2期155-159,共5页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的明确沈阳地区一起小学急性呼吸道感染疫情暴发的病原及其基因进化特征。方法采集2020年12月末沈阳地区某小学暴发疫情中的患病学生咽拭子标本17份,采用TaqMan低密度阵列分析技术(TaqMan low-density arrays,TLDA)进行常见呼吸道病原检测,并对副流感病毒3型阳性标本采用巢式RT-PCR方法核酸扩增、序列测定和系统进化分析。结果TLDA检测确定10份标本为副流感病毒3型核酸阳性,同时伴有条件致病菌感染,扩增HN基因获得7条完整序列。序列系统进化分析表明本研究7个毒株均处于C3a.1进化分支,其核苷酸同源性为99.9%,与原型株Wash/47885/57核苷酸同源性为94.6%,与进化距离最近的浙江株ZJ/11-s-165/KP690785/CHN/11为99.5%。结论引起沈阳地区该起急性呼吸道感染暴发疫情的病原为副流感病毒3型,为我国东北地区首次报道,其处于C3a.1进化分支。Objective To identify the etiology and genetics of the human parainfluenza virus type 3(HPIV3)virus which caused an acute respiratory tract infection outbreak in a primary school in Shenyang.Methods Throat swab samples were collected from 17 students of the primary school where the epidemic of acute respiratory infection outbreak in December 2020 in Shenyang,Liaoning province.TaqMan low-density arrays(TLDA)real-time PCR was performed to simultaneously detect multiple respiratory pathogens.The HN gene was amplified using nested RT-PCR and sequenced,followed by phylogenetic analysis for those HPIV3 positive samples.Results Of the 17 specimens,10 were HPIV3 positive by TLDA Real-time PCR,and were accompanied by conditional pathogen infection,consequently,amplification result ed in 7 complete HN sequences.Phylogenetic analysis showed that the infected HPIV3 virus of the outbreak belonged to HPIV subtype C3a.All the 7 strains detected in this study belonged to subbranch C3a.1 evolutionary branch,with a nucleotide homology of 99.9%,a nucleotide homology of 94.56 with the prototype strain Wash/47885/57 and 99.5%with the most phylogenetically close strain of ZJ/11-s-165/KP690785/CHN/11.Conclusions The HPIV3 virus caused the acute respiratory tract infection outbreak in Shenyang in 2020 and HPIV subtype C3a1 was detected firstly in Northeast China.

关 键 词：副流感病毒 急性呼吸道感染 暴发 

分 类 号：R725.6[医药卫生—儿科] R181.3[医药卫生—临床医学]