模拟高载荷及失重暴露对新西兰大白兔椎间盘影像学与基质金属蛋白酶及其抑制剂表达的相关研究  

Study on the simulated high load and weightlessness exposure on intervertebral discs imaging and matrix metalloporteinase and its inhibitor expression in New Zealand white rabbits

作　　者：陈渲宇 郑超 杜明奎 张振华 季祥 赵辉 靳凯锋 仲伟涛 张黎明 伍骥 Chen Xuanyu;Zheng Chao;Du Mingkui;Zhang Zhenhua;Ji Xiang;Zhao Hui;Jin Kaifeng;Zhong Weitao;Zhang Liming;Wu Ji(Capital Medical University Electric Teaching Hospital,Beijing 100073,China;Department of Orthopedics,Air Force Medical Center,PLA,Beijing 100142,China)

机构地区：[1]首都医科大学电力教学医院骨二科,北京100073 [2]空军特色医学中心骨科,北京100142

出　　处：《中华航空航天医学杂志》2022年第1期8-17,共10页Chinese Journal of Aerospace Medicine

基　　金：国家自然基金项目(81171741)。

摘　　要：目的观察分析模拟高载荷及失重环境下新西兰大白兔脊柱的影像学变化以及椎间盘内基质金属蛋白酶(matrix metalloproteinase,MMP)与基质金属蛋白酶抑制剂(tissue inhibitor of metalloproteinase,TIMP)含量的变化,探讨高载荷及失重环境对椎间盘的影响,为预防和延缓高载荷及失重环境下的腰椎间盘退变提供理论依据。方法采用余数随机单盲法将120只年龄及体重均衡的新西兰大白兔随机分为对照组(30 d、60 d、90 d组)和高载荷/失重组(30 d、60 d、90 d组)共6组,每组20只。通过动物离心机模拟高载荷,尾部悬吊法模拟失重。检测实验动物各实验阶段影像学的改变以及MMP1、MMP3以及TIMP1在椎间盘内的阳性表达率,比较各时间段高载荷/失重组与对照组之间、不同高载荷/失重暴露时间组之间检测结果的统计学差异。结果影像学观测到实验动物腰6骶1椎间盘T2加权像有一定降低。各高载荷/失重组MMP1、MMP3阳性率高于同时间段对照组,差异有统计学意义(t=22.97~145.51,P值均<0.001);MMP1、MMP3阳性率随着暴露时间的延长而增高,不同高载荷/失重暴露时间组之间差异有统计学意义(F=2531.10、1758.80,P值均<0.001)。高载荷/失重组TIMP1阳性率高于同时间段对照组,差异有统计学意义(t=29.34~65.05,P值均<0.001);不同高载荷/失重暴露时间组之间TIMP1阳性率差异有统计学意义(F=462.20,P<0.001)。结论高载荷/失重会引起动物腰椎间盘中MMP1、MMP3以及TIMP1的含量发生改变,且TIMP1早期变化明显,后期敏感性降低。高载荷/失重可能导致椎间盘中MMP与TIMP的含量发生改变,进而引起椎间盘加速退变。Objective To discuss the influence of high load and weightlessness environment on intervertebral disc by observing and analyzing the imaging changes of the spines in New Zealand white rabbits and the content changes of matrix metalloproteinase(MMP)and tissue inhibitor of metalloproteinase(TIMP)in the intervertebral disc under high load and weightlessness environment,and to provide theoretical basis for prevention and delay of lumbar intervertebral disc degeneration under high load and weightlessness environment.Methods A total of 120 New Zealand white rabbits with balanced age and body weight were randomly and averagely divided into control groups(further grouped by 30 d,60 d and 90 d)and high load/weightlessness groups(further grouped by 30 d,60 d and 90 d)with residual single-blind method.High load was simulated by animal centrifuge and weightlessness was simulated by tail suspension.The imaging changes and the positive expression rates of MMP1,MMP3 and TIMP1 in intervertebral disc were detected,and the statistical differences between the high load/weightlessness group and the control group in different time periods were compared,as well as among the different exposure time in load/weightlessness groups.Results From the imaging observation,the T2-weighted images of lumbar 6 sacral 1 intervertebral disc in experimental animals decreased to some extent.The positive rates of MMP1 and MMP3 in each high load/weightlessness group were higher than those in the control group for the same exposure time,and the differences were statistically significant(t=22.97-145.51,all P<0.001).The positive rates of MMP1 and MMP3 increased with the extension of exposure time,and there were statistically differences among the groups with different exposure time in high load/weightlessness group(F=2531.10,1758.80,both P<0.001).The positive rate of TIMP1 in each high load/weightlessness group was higher than that in the control group for the same exposure time,and the difference was statistically significant(t=29.34-65.05,all P<0.001).Th

关 键 词：加速度 超重力 失重模拟 兔 椎间盘 金属蛋白酶类 金属蛋白酶1组织抑制剂 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]