雌激素与跑台运动干预卵巢切除模型小鼠骨量和关节软骨的变化  

Effects of estrogen combined with treadmill exercise on bone mass and articular cartilage in ovariectomized mice

作　　者：黄林科[1,2,3] 韦林华 蒋捷 刘倩 陈蔚蔚[2,3] Huang Linke;Wei Linhua;Jiang Jie;Liu Qian;Chen Weiwei(Department of Orthopedics,The Second Affiliated Hospital of Guangxi Medical University,Nanning 530006,Guangxi Zhuang Autonomous Region,China;Regenerative Medicine Research Center,Department of Orthopedics,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,GuangxiZhuang Autonomous Region,China;Guangxi Key Laboratory of Regenerative Medicine,Nanning 530021,Guangxi Zhuang Autonomous Region,China;Department of Surgery,Nanning Infectious Disease Hospital Affiliated to Guangxi Medical University,Nanning 530023,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]广西医科大学第二附属医院骨科,广西壮族自治区南宁市530006 [2]广西医科大学第一附属医院骨科,再生医学研究中心,广西壮族自治区南宁市530021 [3]广西再生医学重点实验室,广西壮族自治区南宁市530021 [4]广西医科大学附属南宁市传染病医院外科,广西壮族自治区南宁市530023

出　　处：《中国组织工程研究》2023年第8期1166-1171,共6页Chinese Journal of Tissue Engineering Research

基　　金：中国博士后科学基金第62批面上项目(2017M623296XB),项目负责人:陈蔚蔚。

摘　　要：背景:绝经后妇女易发生骨质疏松和骨关节炎,雌激素替代治疗联合跑台运动对骨量和关节软骨的影响尚不明确。目的:探索外源性雌激素联合低强度跑台运动对卵巢切除小鼠骨量和关节软骨的影响。方法:35只C57BL/6雌性小鼠随机均分为假手术组、假手术+运动组、卵巢切除组、雌激素组以及雌激素+运动组,根据分组情况对小鼠进行双侧卵巢切除术或假手术,雌激素组和雌激素+运动组术后隔天注射雌激素,假手术+运动组和雌激素+运动组小鼠进行6周的跑台运动。观察小鼠股骨远端干骺端的破骨细胞数量和骨量变化,采用国际骨关节炎研究学会评分评估小鼠膝关节软骨改变情况。结果与结论:①雌激素+运动组小鼠的骨体积分数较卵巢切除组显著升高(P<0.01),与假手术组、假手术+运动组和雌激素组比较差异无显著性意义(P>0.05);②雌激素+运动组小鼠的骨表面破骨细胞数量和骨表面破骨细胞面积较卵巢切除组降低(P<0.01),与假手术+运动组和雌激素组相比差异无显著性意义(P>0.05);③雌激素+运动组小鼠的膝关节国际骨关节炎研究学会评分较其他组显著升高(P<0.01);④提示雌激素联合低强度跑台运动可提升卵巢切除小鼠的骨量,但会导致关节软骨的退行性变。BACKGROUND:Postmenopausal women are prone to developing osteoporosis and osteoarthritis,and the effect of estrogen replacement therapy combined with treadmill exercise on bone mass and articular cartilage is unclear.OBJECTIVE:To investigate the effects of exogenous estrogen combined with low intensity treadmill exercise on bone mass and articular cartilage in an ovariectomized mouse model.METHODS:Thirty-five C57BL/6 mice were randomly divided into five groups:sham group,sham and low intensity treadmill exercise group(sham-sport group),ovariectomized group,ovariectomized and supplying exogenous estrogen group(estrogen group),ovariectomized and supplying exogenous estrogen combined with low intensity treadmill exercise group(estrogen-sport group).The mice were subjected to bilateral ovariectomy or sham operation.The mice in the estrogen and estrogen-sport groups were injected with estrogen every other day after the operation,and the mice in the sham-sport and estrogensport groups ran on a treadmill for 6 weeks.The changes in the number of osteoclasts and bone mass in the metaphysis of the distal femur of the mice were observed.Osteoarthritis Research Society International histopathology was used to evaluate the degeneration of knee joint cartilage in mice.RESULTS AND CONCLUSION:Bone volume per total volume in the estrogen-sport group was significantly higher than that in the ovariectomized group(P<0.01).There was no statistical difference among the estrogen-sport,sham,sham-sport group,and estrogen groups(P>0.05).Osteoclast number per bone surface and osteoclast surface per bone surface were significantly decreased in the estrogen-sport group compared with the ovariectomized group(P<0.01).There was no statistical significance among the estrogen-sport,sham-sport,and estrogen groups(P>0.05).The knee Osteoarthritis Research Society International histopathology of the estrogen-sport group was significantly higher than that of the other groups(P<0.01).In conclusion,exogenous estrogen supplementation with low-intensity tr

关 键 词：雌激素 骨量 破骨细胞 跑台运动 卵巢切除小鼠 软骨 骨关节炎 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R875