叉头样转录因子3在粪肠球菌感染模型大鼠难治性根尖周炎病变中的表达  

Expression of forkhead box protein 3 in refractory periapical periodontitis in rats with Enterococcus faecalis infection

作　　者：李梦菲 仉红 赵少剑 殷广浩 王其宝[2] Li Mengfei;Zhang Hong;Zhao Shaojian;Yin Guanghao;Wang Qibao(School of Stomatology,Binzhou Medical University,Yantai 264000,Shandong Province,China;Second Department of Dentistry and Endodontics,JinanStomatological Hospital,Jinan 250000,Shandong Province,China)

机构地区：[1]滨州医学院口腔医学院,山东省烟台市264000 [2]济南市口腔医院牙体牙髓病二科,山东省济南市250000

出　　处：《中国组织工程研究》2023年第8期1187-1192,共6页Chinese Journal of Tissue Engineering Research

基　　金：山东省医药卫生科技发展计划(2018ws483),项目负责人:王其宝。

摘　　要：背景:粪肠球菌作为优势菌的难治性根尖周炎是一种经过反复多次的根管治疗之后仍然伴随着持续性炎症和骨吸收的疾病。多种实验表明,叉头样转录因子3(forkhead box protein 3,Foxp3)通过影响机体自身免疫反应参与各种炎症疾病。目的:观察Foxp3在粪肠球菌感染的大鼠难治性根尖周炎病变中的表达情况。方法:6周龄SD大鼠32只,随机各挑选16只分成未封菌组和封菌组。将32只大鼠在无痛条件下行双侧下颌第1磨牙中央窝开髓,未封菌组放入PBS小棉球,封菌组放入粪肠球菌混悬液小棉球,随后在双侧内髓腔放置流动树脂后光固化。常规水食室温饲养,于1,2,3,4周两组各随机选取4只大鼠取下颌骨组织;并以0周大鼠未做开髓处理为空白对照组。用苏木精-伊红染色观察大鼠根尖周部位的炎症组织病理变化;RT-PCR和免疫组织化学检测根尖周组织Foxp3的表达变化。结果与结论:①苏木精-伊红染色显示:未封菌组、封菌组大鼠根尖周炎的动物模型均建立成功,根尖周围组织区域均可见到明显的炎性细胞的浸润;②RT-PCR结果显示:Foxp3在未封菌组根尖周组织区域的mRNA表达水平呈现1-4周逐步递增;封菌组根尖区组织Foxp3的mRNA表达水平在3周达到高峰后下降,在2,4周与未封菌组比较,差异有显著性意义(P<0.05);③免疫组织化学结果显示:未封菌组中Foxp3的阳性细胞表达量随着时间递增,封菌组在第3周表达高峰后第4周下降,与RT-PCR的结果一致(P<0.05);④结论:Foxp3参与慢性根尖周炎和难治性根尖周炎的进展过程;在炎症慢性期,Foxp3在难治性根尖周炎中表达明显降低,提示其可能与粪肠球菌有一定的关系。BACKGROUND:Refractory periapical periodontitis,with Enterococcus faecalis as a dominant bacterium,is a disease that continues with persistent inflammation and bone resorption after repeated and multiple root canal treatments.Multiple experiments have shown that forkhead box protein 3(Foxp3)is involved in various inflammatory diseases by influencing the body’s autoimmune response.OBJECTIVE:To observe the expression of Foxp3 in refractory periapical periodontitis in Enterococcus faecalis-infected rats.METHODS:Thirty-three Sprague-Dawley rats at the age of 6 weeks were selected as experimental objects,32 of which were randomly selected and divided into control and experimental groups.These 32 rats were submitted to open pulp in the central fossa of bilateral mandibular first molars under painless conditions.PBS pellets and Enterococcus faecalis suspension pellets were placed into the pulp in the control and experimental groups,respectively,followed by light curing after placing flowing resin in the bilateral inner medullary cavities.At 1,2,3,4 weeks after regular water and food feeding in room temperature,four rats from each group were randomly selected for euthanasia.Rats aged 0 week without dental pulp opening were used as blank controls.Hematoxylin-eosin staining was to observe the inflammatory histopathological changes in the experimental periapical sites of rats.RT-PCR and immunohistochemical detection were used to detect the expression of Foxp3 in the periapical tissue.RESULTS AND CONCLUSION:Hematoxylin-eosin staining results showed that:the animal models of periapical periodontitis were successfully established in the control and experimental groups,and obvious inflammatory cell infiltration was seen in the periapical tissue.The results of RT-PCR showed that the mRNA expression level of Foxp3 in the periapical tissue in the control group rose in a stepwise manner from 1 to 4 weeks,while the mRNA expression level of Foxp3 in the periapical tissue in the experimental group decreased after peaking at 3 weeks(P

关 键 词：慢性根尖周炎 难治性根尖周炎 粪肠球菌 FOXP3 大鼠 

分 类 号：R446[医药卫生—诊断学] R496[医药卫生—临床医学] R318