海藻酸盐支架修复关节软骨  

Alginate scaffold in articular cartilage repair

作　　者：李诚 郑国爽 蒯贤东 于炜婷 Li Cheng;Zheng Guoshuang;Kuai Xiandong;Yu Weiting(Zhongshan Clinical College of Dalian University,Dalian 116001,Liaoning Province,China;Department of Orthopedics,Affiliated Zhongshan Hospital of Dalian University,Dalian 116001,Liaoning Province,China)

机构地区：[1]大连大学中山临床学院,辽宁省大连市116001 [2]大连大学附属中山医院骨科,辽宁省大连市116001

出　　处：《中国组织工程研究》2023年第7期1080-1088,共9页Chinese Journal of Tissue Engineering Research

基　　金：辽宁省重点研发计划项目(2020JH2/10300093),负责人:于炜婷;辽宁省高等学校创新人才支持计划项目(辽教函[2018]478号),负责人:于炜婷;辽宁省“百千万人才工程”资助项目(辽人社[2019]45号),负责人:于炜婷。

摘　　要：背景:由于关节软骨的解剖、生理特点,其自我修复能力有限,故而如何修复大面积的软骨缺损(直径>4 mm)成为医学界备受瞩目的问题之一。伴随生物材料和组织工程学科的发展,通过支架技术尤其具有模拟细胞外基质微环境的水凝胶支架研究的深入发展,为软骨损伤修复提供了新的治疗思路。目的:就海藻酸盐的性质、海藻酸盐水凝胶支架的制备以及在软骨损伤修复中的研究进展进行综述。方法:利用计算机检索Web of Science、PubMed、万方和中国知网数据库,中文检索词为“海藻酸盐水凝胶、软骨组织工程或软骨、骨软骨、支架”,英文检索词为“alginate hydrogel,cartilage tissue engineering or chondro*,osteochondral,scaffold”,检索文献时间范围2016年1月至2020年12月。最终按入组标准筛选后纳入60篇文献进行综述分析。结果与结论:①海藻酸盐作为带负电荷的天然亲水性多糖,可通过修饰技术赋予传统海藻酸盐水凝胶更优良的机械性能、黏附性、生物降解性以及生物相容性等。②修饰后的海藻酸盐复合水凝胶支架利于维持种子细胞的正常形态、合成相应的细胞外基质,促进成软骨相关基因的表达,表现出优良的成软骨能力。③有报道证实海藻酸盐复合水凝胶支架可在动物体内形成与周边正常软骨相似的软骨组织,有效修复缺损部位。④因此,海藻酸盐复合水凝胶支架具备良好的促软骨修复能力,为软骨组织工程提供新的治疗思路,但该材料未来还需更完善的临床前试验数据的支撑,以推进其临床转化进程。BACKGROUND:Due to the anatomical and physiological characteristics of articular cartilage,its self-repair ability is limited,so how to repair large cartilage defects(diameter>4 mm)has become one of the most atiractive problems in the medical field.With the development of bio-materials and tissue engineering,the further development of scaffold technology,especially the hydrogel scaffold that simulates the microenvironment of extracellular matrix,provides a new therapeutic idea for the repair of cartilage injury.OBJECTIVE:To summarize the properties of alginate,the preparation of alginate saline gel scaffold and the research progress in the repair of cartilage injury.METHODS:A computer-based search of Web of Science,PubMed,Wanfang and CNKI was performed for papers published from January 2016 to December 2020 with the key words of“alginate hydrogel;cartilage tissue engineering or chondro*,osteochondral,scaffold”in Chinese and English.According to the inclusion criteria,60 articles were included for review.RESULTS AND CONCLUSION:(1)Alginate,as negatively charged natural hydrophilic polysaccharide,can endow traditional alginate saline gel with betier mechanical properties,adhesion,biodegradability and biocompatibility by modification technology.(2)The modified alginate composite hydrogel scaffold is beneficial to maintain the normal morphology of seed cells,synthesize the corresponding extracellular matrix,promote the expression of cartilage-related genes,and show excellent chondrogenic ability.(3)It has been reported that alginate composite hydrogel scaffold can form cartilage tissue similar to the surrounding normal cartilage in animals and effectively repair the defect.(4)In summary,alginate composite hydrogel scaffold has a good ability to promote cartilage repair,which provides a new therapeutic idea for cartilage tissue engineering.However,this material needs to be supported by more perfect preclinical trial data to promote its clinical transformation process.

关 键 词：海藻酸盐 软骨 骨软骨 支架 组织工程 修饰 水凝胶 综述 

分 类 号：R459.9[医药卫生—治疗学] R363[医药卫生—临床医学] R364