一株微藻附生菌的分离鉴定及藻菌体系的氮吸收特性  

Isolation and Identification of Symbiotic Bacteria from Oocystis borgei and Characteristics of Nitrogen Uptake in Algae-Bacteria System

作　　者：陈永志 黄翔鹄[1] 朱春华[1] 莫峰 温其交 张玉蕾 CHEN Yong-zhi;HUANG Xiang-hu;ZHU Chun-hua;MO Feng;WEN Qi-jiao;ZHANG Yu-lei(College of Fisheries,Guangdong Ocean University,Zhanjiang 524088,China;Lianjiang Shrimp Group Co.,Ltd.,Zhanjiang 524499,China)

机构地区：[1]广东海洋大学水产学院,广东湛江524088 [2]廉江市养虾集团有限公司,广东湛江524499

出　　处：《广东海洋大学学报》2022年第4期49-56,共8页Journal of Guangdong Ocean University

基　　金：国家重点研发计划“蓝色粮仓科技创新”重点专项(2020YFD0900205);广东海洋大学科研启动经费资助项目(060302022102);广东省现代农业产业技术体系创新团队建设专项资金(2019KJ149);对虾养殖产业人才发展项目(B21341)。

摘　　要：【目的】优化水产养殖体系中的藻菌群落,合理构建藻菌体系,提高微藻和细菌对养殖水体中氮的去除效果。【方法】从稳定培养的波吉卵囊藻藻际环境中分离到1株附生细菌OA-1,通过16S rDNA测序、形态学观察和生化试验对该菌进行鉴定。构建波吉卵囊藻和菌株OA-1的藻菌体系,分析该体系对不同氮源的吸收速率。【结果与结论】经鉴定,菌株OA-1为居海噬冷菌(Algoriphagus marincola)。该细菌可促进波吉卵囊藻的生长,加菌条件下培养10 d时,波吉卵囊藻叶绿素a质量浓度达到2.74 mg/L,比对照组增加45.74%,显著高于纯藻培养对照组(P<0.05)。藻菌体系对氮的吸收速率显著高于纯藻体系和纯菌体系(P<0.05),在以亚硝酸钠和氯化铵为氮源时,藻菌间存在协同作用。藻菌体系对硝酸钠、亚硝酸钠、氯化铵和尿素的吸收速率最高分别为36.93、6.28、20.09和63.58μmol·h^(-1)·g^(-1);以硝酸钠为氮源时,藻菌体系的氮吸收速率随浓度的升高而升高;以亚硝酸钠为氮源时,氮浓度为3 mmol·L^(-1)时出现吸收拐点;以氯化铵或尿素为氮源时,氮浓度为5 mmol·L^(-1)时出现吸收拐点。构建的藻菌体系可显著增强对氮的吸收利用,可将该藻菌体系开发应用于养殖水环境调控。【Objective】 To optimize the algae and bacteria community in the aquaculture system, and construct the algae-bacteria system reasonably, and improve the removal effect of microalgae and bacteria on nitrogen in aquaculture water. 【Method】A symbiotic strain OA-1 was isolated from phycosphere of Oocystis borgei. Identification of the symbiotic strain OA-1 was carried out by 16S r DNA sequencing,morphological observation and biochemical test. Then, the co-culture system of O. borgei and OA-1 strain was constructed, and the absorption rate of different nitrogen sources in the system was analyzed.【Result】 The OA-1 strain was identified as Algoriphagus marincola. The co-cultivation of algae and bacteria promoted the growth of algae, and chlorophyll a content reached 2.74 mg/L on the 10th day with bacteria added, which was 45.74% higher than that of the control group. The highest absorption rates of sodium nitrate, sodium nitrite, ammonium chloride and urea by the algal bacterial system were 36.93,6.28, 20.09 and 63.58 μmol·h^(-1)·g^(-1), respectively. When sodium nitrate was used as nitrogen source, the nitrogen absorption rate of the algal bacteria system increased with the increase of the concentration.When the nitrogen source was sodium nitrite, the inflection point appeared at 3 mmol·L^(-1), while ammonium chloride or urea was used, the inflection point was 5 mmol·L^(-1). 【Conclusion】The combined system could significantly enhance the absorption and utilization of nitrogen. Therefore, the algae-bacteria system could be developed and applied to the regulation of aquaculture water environment.

关 键 词：居海噬冷菌 波吉卵囊藻 藻菌体系 氮 吸收速率 

分 类 号：S917.1[农业科学—水产科学]