湛江等鞭金藻多肽AYAPE对UVB诱导的HaCaT细胞抗光老化活性的影响  

Effects of Bioactivity of AYAPE, A Peptide Isolated from Isochrysis zhanjiangensis,on UVB-induced HaCaT Cells

作　　者：何苑琳 郑兆婉 周春霞 洪鹏志 HE Yuan-lin;ZHENG Zhao-wan;ZHOU Chun-xia;HONG Peng-zhi(College of Food Science and Technology,Guangdong Ocean University/Guangdong Key Laboratory of aquatic product processing and safety/Guangdong Provincial Engineering Technology Research Center of Marine Food/Guangdong Modern Agricultural Science and Technology Innovation Center/Guangdong Province Engineering Laboratory for Marine Biological Products,Zhanjiang 524088,China;School of Chemistry and Environmental Science,Guangdong Ocean University,Zhanjiang 524088,China;Collaborative Innovation Center of seafood Deep Processing,Dalian Polytechnic University,Dalian 116034,China)

机构地区：[1]广东海洋大学食品科技学院/广东省水产品加工与安全重点实验室/广东省海洋食品工程技术研究中心/广东省现代农业科技创新中心/广东省海洋生物制品工程实验室,广东湛江524088 [2]广东海洋大学化学与环境学院,广东湛江524088 [3]海洋食品精深加工关键技术省部共建协同创新中心,大连工业大学,辽宁大连116034

出　　处：《广东海洋大学学报》2022年第4期111-117,共7页Journal of Guangdong Ocean University

基　　金：广东省现代农业产业技术体系创新团队建设项目(2021KJ150);南方海洋科学与工程重点实验室(湛江)资助项目(ZJW-2019-07);湛江市海洋经济创新发展示范市建设项目(XM-202008-01B1)。

摘　　要：【目的】探究从湛江等鞭金藻(Isochrysis zhanjiangensis)中提取的一种五肽AYAPE对UVB照射引起的人永生化角质形成细胞(HaCaT)的抗光老化活性的影响。【方法】CCK-8法测定细胞活力,酶联免疫吸附法ELISA试剂盒测定MMP-1蛋白和Ⅰ型前胶原的分泌变化,免疫组化染色测定p53蛋白的核内表达情况,蛋白质免疫印迹法检测NF-κB信号转导通路中p65蛋白磷酸化的表达及Caspase-3和Caspase-9蛋白的激活程度。【结果】低、中、高浓度下的多肽AYAPE对HaCaT细胞均无毒害作用,且可提高被UVB辐射所损伤的细胞活力;与空白组相比,对照组在UVB的辐射下明显NF-κB信号转导通路被激活,引起细胞凋亡;与对照组相比,样品组中NF-κB信号通路蛋白p65、MMP-1、Cleaved Caspase-3和Cleaved Caspase-9的表达明显降低,而Ⅰ型前胶原的表达明显增加(P<0.05)。【结论】多肽AYAPE通过有效抑制NF-κB信号通路激活、MMP-1分泌、胶原蛋白降解,并显著抑制HaCaT细胞凋亡。【Objective】 To investigate the anti-photoaging activity of a peptide AYAPE isolated from Isochrysis zhanjiangensis on human immortalized keratinocytes(HaCaT) induced by UVB irradiation.【Method】 Cell viability was measured by CCK-8 method. The secretion of MMP-1 protein and procollagen Ⅰ were measured by ELISA kit. The nuclear expression of p53 protein was measured by immunohistochemical staining. The activation of Caspase-3 and Caspase-9 proteins, and the expression of phosphorylated p65 protein in NF-κB signaling pathways were measured by western blot. 【Result】The peptide AYAPE at low, medium and high concentrations had no toxic effect on HaCaT cells, and could improve the cell viability damaged by UVB radiation. Compared with the blank group, the control group significantly activated NF-κB transduction pathway exposed to UVB caused apoptosis.Compared with the control group, the expression of p-p65, MMP-1, Cleaved Caspase-3 and Cleaved Caspase-9 decreased significantly by AYAPE treatment, but the expression of procollagen Ⅰ increased significantly.(P < 0.05). 【Conclusion】 Peptide AYAPE can effectively inhibit NF-κB signaling pathway, MMP-1 secretion, collagen degradation and the apoptosis of HaCaT cells.

关 键 词：HACAT 抗光老化 UVB Caspase-3 CASPASE-9 

分 类 号：P745[天文地球—海洋生物学]