血肿抽吸术联合高压氧治疗对脑出血大鼠脑组织的保护作用  

Protective effects of hematoma aspiration combined with hyperbaric oxygen therapy on rat brain tissue with cerebral hemorrhage

作　　者：周新管 王磊 任登鹏 杨世峰 李太红 Zhou Xinguan;Wang Lei;Ren Dengpeng;Yang Shifeng;Li Taihong(Department of Neurosurgery,Yuncheng Central Hospital,Yuncheng 044000,China)

机构地区：[1]运城市中心医院神经外科,运城044000

出　　处：《中华航海医学与高气压医学杂志》2022年第1期93-98,共6页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

摘　　要：目的探讨血肿抽吸术联合高压氧治疗对脑出血大鼠脑组织的保护作用。方法40只大鼠随机分为假手术组、模型组、手术组(血肿抽吸术)和联合治疗组(微创血肿抽吸术+高压氧),每组10只。通过Longa评分法对各组大鼠神经功能进行评分,干湿重量法检测各组大鼠脑组织含水量,HE染色法观察各组大鼠脑海马组织病理变化情况,TUNEL法检测各组大鼠脑海马神经细胞凋亡数目,qRT-PCR和Western blotting法检测各组大鼠脑海马组织中脑源性神经营养因子(BDNF)、水通道蛋白4(AQP4)的表达水平,Western blotting法检测各组大鼠脑组织Bax、Bcl-2、JAK2、p-JAK2、STAT3、p-STAT3蛋白表达水平。结果与假手术组相比,模型组大鼠神经功能评分、脑组织含水量显著升高,脑组织出血,细胞固缩、间隙变大,胶质细胞增多,神经元减少;海马神经细胞凋亡水平升高(P<0.05);AQP4和p-JAK2、p-STAT3表达升高,而BDNF表达下降(P<0.05)。与模型组相比,手术组和联合治疗组大鼠神经功能评分、脑组织含水量下降,脑组织损伤情况均得到改善;海马神经细胞凋亡水平降低(P<0.05);AQP4、p-JAK2和p-STAT3表达下降,而BDNF表达升高(P<0.05)。与手术组相比,联合治疗组神经功能评分、脑组织含水量显著下降,细胞固缩、水肿等现象明显消退,海马神经细胞凋亡水平降低(P<0.05);AQP4、p-JAK2和p-STAT3表达下降,而BDNF表达升高(P<0.05)。结论血肿抽吸术联合高压氧治疗对脑出血大鼠脑损伤具有保护作用,其机制可能与JAK2/STAT3信号通路被抑制有关。Objective To explore the protective effects of hematoma aspiration combined with hyperbaric oxygen(HBO)therapy on the brain tissue of rats after cerebral hemorrhage.Methods A total of 40 rats were randomly divided into four groups:sham operation group,model group,operation group(hematoma aspiration),and combined treatment group(minimally invasive hematoma aspiration+HBO),with 10 rats in each group.The neurological deficits of rats in each group were scored by Longa scoring method.The water content of the brain tissue of rats in each group was detected by dry/wet weight measurement.The pathological changes in the hippocampus tissue of rats in each group were observed by H&E staining.The number of hippocampal neuronal apoptosis of rats in each group was detected by TUNEL assay.The expression levels of brain derived neurotrophic factor(BDNF)and Aquaporin-4(AQP4)in the hippocampus of rats in each group were detected by qRT-PCR and Western blotting.The protein expressions of Bax,Bcl-2,JAK2,p-JAK2,STAT3,and p-STAT3 in the brain tissue of rats in each group were detected by Western blotting.Results Compared with the sham operation group,the model group had significantly higher nerve injury function scores and brain tissue water content,and showed brain tissue hemorrhage,cell pyknosis,enlarged intercellular space,increased glial cells,and decreased neurons;the apoptosis level of hippocampal neurons in the model group increased(P<0.05);the AQP4,p-JAK2,and p-STAT3 expressions in the model group increased,while the BDNF expression in the model group decreased(P<0.05).Compared with the model group,the operation group and the combined treatment group had lower nerve injury function scores and less brain tissue water content,and the brain tissue injury in the two groups was reduced.In both groups,the apoptosis level of hippocampal neurons decreased(P<0.05);the AQP4,p-JAK2,and p-STAT3 expressions decreased,while the BDNF expression increased(P<0.05).Compared with the operation group,the combined treatment group had significantl

关 键 词：血肿抽吸术 高压氧 脑出血 JAK2/STAT3信号通路 

分 类 号：R651.12[医药卫生—外科学]