空气喷射换热在PCR控温中的实验研究  

Experimental study of thermal management for PCR instrument with air injection

作　　者：马杰[1] 廖全[1] 肖育劲 Ma Jie;Liao Quan;Xiao Yujin(College of Energy and Power Engineering,Chongqing University,Chongqing 400044,China;Shenzhen YHLO Biotechnology Co.,Ltd,Shenzhen 518000,China)

机构地区：[1]重庆大学能源与动力工程学院,重庆400044 [2]深圳市亚辉龙生物科技股份有限公司,深圳518000

出　　处：《现代仪器与医疗》2022年第3期50-55,60,共7页Modern Instruments & Medical Treatment

摘　　要：目的实现对微型扩增腔快速、准确的温度循环控制.方法采用空气喷射换热方式对微型扩增腔内扩增液的PCR过程进行热管理,通过搭建风速及风温可调的空气喷射换热PCR温度控制实验平台,充分利用空气射流冲击的高效换热特性,强化空气与扩增液间的传热过程.结果获得了风速、风温对微型扩增腔内不同扩增介质升降温速率的影响规律,且获得了较快的升降温速率.结论为空气喷射换热方式在PCR循环热管理中的实践应用提供了参考数据,也为我国PCR控温方式的研究提供了新思路.Objective Achieving the rapid and accurate temperature cycling control of the micro amplification chamber.Methods An air jet heat transfer method was used to thermally manage the PCR process of the amplification solution in the micro amplification chamber,and by building an air jet heat transfer PCR temperature control experimental platform with adjustable wind speed and temperature,which make full use of the high-efficiency heat transfer characteristics of air jet impact and strengthen the heat transfer process between air and amplification fluid.Results The effect of wind speed and wind temperature on the heating and cooling rates of different amplification media in the micro-amplification chamber was obtained,and a faster heating and cooling rate was obtained.Conclusion This study provides reference data for the practical application of air jet heat transfer in the thermal management of PCR cycles,and also provides new ideas for the study of PCR temperature control methods in my country.

关 键 词：PCR仪 空气喷射换热 微型扩增腔 热管理 升降温速率 

分 类 号：TK519[动力工程及工程热物理—热能工程]