干湿生化分析仪检测电解质的可比性研究  

Comparability of electrolyte detection by wet and dry biochemical analyzer

作　　者：刘曼 宋志新 陈大鹏[1] LIU Man;SONG Zhixin;CHEN Dapeng(Department of Clinical laboratory,Children's Hospital of Chongqing Medical University/National Clinical Research Center for Child Health and Disorders/Ministry of Education Key Laboratory of Child Development and Disorders/Chongqing Key Laboratory of Pediatrics,Chongqing 400010,China)

机构地区：[1]重庆医科大学附属儿童医院医学检验科/国家儿童健康与疾病临床医学研究中心/儿童发育疾病研究教育部重点实验室/儿科学重庆市重点实验室,重庆400010

出　　处：《现代医药卫生》2022年第13期2161-2164,2177,共5页Journal of Modern Medicine & Health

基　　金：国家自然科学基金项目(81801956)。

摘　　要：目的探讨干式生化分析仪与传统湿化学分析仪检测电解质结果的可比性。方法参照CLSI EP9-A3文件开展方法学比对,参考系统选择罗氏Cobas c701型全自动生化分析仪,奥森多V5600型全自动生化分析仪作为待测系统,2个系统(干式生化分析仪与传统湿化学分析仪)分别测定40份临床标本。采用广义极端学生化偏差(ESD)法检测离群值,绘制偏差图和散点图判断数据特点。利用偏差图初步估算偏倚,拟合回归方程并计算医学决定水平处的偏差。结果ESD法检查未找到离群值点。2个系统测定结果相关性良好(R^(2)≥0.95),各项目偏倚均小于可接受标准。结论干式生化分析仪与传统湿化学分析仪检测电解质结果无明显差异,具有可比性。Objective To discuss the comparability of electrolyte detection results by dry biochemical analyzer and traditional wet chemical analyzer.Methods The methodological comparison was carried out according to the Clinical and Laboratory Standards Institute(CLSI)EP9-A3 document,the Roche Cobasc701 automatic biochemical analyzer was selected as the reference system,and the Ortho VITROS 5600 automatic biochemical analyzer was selected as the under-test system,and a total of 40 clinical specimens were measured respectively by two systems(dry biochemical analyzer and traditional wet chemistry analyzer).The generalized extreme studenization deviate(ESD)method was used to detect outliers,and bias and scatter plots were plotted to determine the characteristics of the data.The bias was preliminarily estimated by using the deviation diagram,and the regression equation was fitted and the deviation at the medical decision level was calculated.Results The outlier was no found by the ESD method.The results of the two systems were well correlated(R^(2)≥0.95),and the bias of each item was less than the acceptable standard.Conclusion There was no obvious difference between dry biochemical analyzer and traditional wet chemical analyzer in the results of electrolyte detection,which is comparable.

关 键 词：电解质 方法学 可比性 偏倚分析 

分 类 号：R446.1[医药卫生—诊断学]