Prostate-derived IL-1βupregulates expression of NMDA receptor in the paraventricular nucleus and shortens ejaculation latency in rats with experimental autoimmune prostatitis  

作　　者：Jie Yang Jiao-Chen Luan Jian-Huai Chen Qi-Jie Zhang Jian-Xin Xue Ya-Min Wang Guo-Qing Zhu Ning-Hong Song Zeng-Jun Wang Jia-Dong Xia 

机构地区：[1]Department of Urology,First Affiliated Hospital of Nanjing Medical University,Nanjing 210008,China [2]Department of Andrology,Jiangsu Province Hospital of Chinese Medicine,Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210008,China [3]Key Laboratory of Cardiovascular Disease and Molecular Intervention,Department of Physiology,Nanjing Medical University,Nanjing 210008,China

出　　处：《Asian Journal of Andrology》2022年第2期213-218,共6页亚洲男性学杂志（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(Grant No.81501245 and No.81971377);Fellowship of China Postdoctoral Science Foundation(Grant No.2020M671393);Jiangsu Province Postdoctoral Research Support Project(Grant No.2020Z134).

摘　　要：Experimental autoimmune prostatitis(EAP)-induced persistent inflammatory immune response can significantly upregulate the expression of N-methyl-D-aspartic acid(NMDA)receptors in the paraventricular nucleus(PVN).However,the mechanism has not yet been elucidated.Herein,we screened out the target prostate-derived inflammation cytokines(PDICs)by comparing the inflammatory cytokine levels in peripheral blood and cerebrospinal fluid(CSF)between EAP rats and their controls.After identifying the target PDIC,qualified males in initial copulatory behavior testing(CBT)were subjected to implanting tubes onto bilateral PVN.Next,they were randomly divided into four subgroups(EAP-1,EAP-2,Control-1,and Control-2).After 1-week recovery,EAP-1 rats were microinjected with the target PDIC inhibitor,Control-1 rats were microinjected with the target PDIC,while the EAP-2 and Control-2 subgroups were only treated with the same amount of artificial CSF(aCSF).Results showed that only interleukin-1β(IL-1β)had significantly increased mRNA-expression in the prostate of EAP rats compared to the controls(P<0.001)and significantly higher protein concentrations in both the serum(P=0.001)and CSF(P<0.001)of the EAP groups compared to the Control groups.Therefore,IL-1βwas identified as the target PDIC which crosses the blood-brain barrier,thereby influencing the central nervous system.Moreover,the EAP-1 subgroup displayed a gradually prolonged ejaculation latency(EL)in the last three CBTs(all P<0.01)and a significantly lower expression of NMDA NR1 subunit in the PVN(P=0.043)compared to the respective control groups after a 10-day central administration of IL-1βinhibitors.However,the Control-1 subgroup showed a gradually shortened EL(P<0.01)and a significantly higher NR1 expression(P=0.004)after homochronous IL-1βadministration.Therefore,we identified IL-1βas the primary PDIC which shortens EL in EAP rats.However,further studies should be conducted to elucidate the specific molecular mechanisms through which IL-1βupregulates NMDA expression.

关 键 词：chronic prostatitis N-methyl-D-aspartic acid receptor paraventricular nucleus premature ejaculation prostate-derived inflammation cytokines sympathetic nervous system 

分 类 号：R697.3[医药卫生—泌尿科学]