Evaluation of the sperm DNA fragmentation index in infertile Japanese men by in-house flow cytometric analysis  

作　　者：Akiyoshi Osaka Hiroshi Okada Sae Onozuka Takashi Tanaka Toshiyuki Iwahata Yukihito Shimomura Yoshitomo Kobori Kazutaka Saito Kouhei Sugimoto 

机构地区：[1]Department of Urology,Dokkyo Medical University Saitama Medical Center,2-1-50 Minamikoshigaya,Koshigaya,Saitama 343-8555,Japan [2]International Center for Reproductive Medicine,Dokkyo Medical University Saitama Medical Center,2-1-50 Minamikoshigaya,Koshigaya,Saitama 343-8555,Japan

出　　处：《Asian Journal of Andrology》2022年第1期40-44,共5页亚洲男性学杂志（英文版）

基　　金：The authors received funding from the Japan Agency for Medical Research and Development(AMED,grant No.17930555)for this work.

摘　　要：Semen analysis has long been used to evaluate male fertility.Recently,several sperm function tests have been developed.Of those,the sperm DNA fragmentation index(DFI),which describes the status of the sperm DNA,is thought to be a suitable parameter for evaluating male fertility.However,there have been no large-scale studies on the sperm DFI of Japanese men.Therefore,we investigated the feasibility of using an in-house flow cytometry-based sperm DFI analysis based on the sperm DNA fragmentation test of sperm chromatin structure assay(SCSA)to assess male fertility in Japan.This study enrolled 743 infertile and 20 fertile Japanese men.To evaluate reproducibility,inter-and intraobserver precision was analyzed.A receiver operating characteristic curve analysis was used to set a cutoff value for the sperm DFI to identify men who could father children by timed intercourse or intrauterine insemination.The variability of the sperm DFI among fertile volunteers was determined.The relationship between semen parameters and the sperm DFI was assessed by Spearman’s rho test.A precision analysis revealed good reproducibility of the sperm DFI.The cutoff value of sperm DNA fragmentation in infertile men was 24.0%.Semen volume had no relationship with the sperm DFI.Sperm concentration,sperm motility,total motile sperm count,and percentage of normal-shaped sperm were significantly and negatively correlated with the sperm DFI.The median sperm DFI was smaller in fertile volunteers(7.7%)than that in infertile men(19.4%).Sperm DNA fragmentation analysis can be used to assess sperm functions that cannot be evaluated by ordinary semen analysis.

关 键 词：DNA fragmentation flow cytometry male infertility semen analysis SPERMATOZOA 

分 类 号：R697[医药卫生—泌尿科学]