电针对膝骨关节炎大鼠脊髓背角CB2R、p-P38和p-ERK表达的影响  

Effect of Electroacupuncture on Expression of CB2R,p-P38,p-ERK in Spinal Dorsal Horn of Rats with Knee Osteoarthritis

作　　者：李杨乐 杨锋[2] 樊静杰 郑晓慧 朱芮 郑洁 LI Yangle;YANG Feng;FAN Jingjie;ZHENG Xiaohui;ZHU Rui;ZHENG Jie(Institution of Acupuncture-moxibustion and Massage,Shaanxi University of Chinese Medicine,Xianyang 712046,China;First Clinical Medical College,Shaanxi University of Chinese Medicine,Xianyang 712046,China;Shaanxi Key Laboratory of Acupuncture and Herbal Medicine,Xianyang 712046,China)

机构地区：[1]陕西中医药大学针灸推拿学院,陕西咸阳712046 [2]陕西中医药大学第一临床医学院,陕西咸阳712046 [3]陕西省针药结合重点实验室,陕西咸阳712046

出　　处：《中国中医药信息杂志》2022年第7期60-66,共7页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81804162);陕西省教育厅青年创新团队科研项目(21JP034);陕西高校青年创新团队建设项目(2019年);陕西中医药大学学科创新团队建设项目(2019-YL02)。

摘　　要：目的观察电针对膝骨关节炎(KOA)大鼠痛性行为学及脊髓背角大麻素受体2(CB2R)、p-P38、p-ERK表达的影响,探讨电针治疗KOA慢性疼痛的作用机制。方法48只SD大鼠随机分为空白组、盐水组、模型组、电针组、CB2R阻断组、CB2R阻断+电针组,每组8只。除空白组和盐水组外,采用左膝关节腔注射谷氨酸钠碘乙酸制备KOA慢性疼痛模型,盐水组左膝关节腔注射生理盐水。注射后第15日,电针组于左侧“阳陵泉”“内膝眼”行电针刺激,15 min/次,1次/d,CB2R阻断组腹腔注射CB2R阻断剂,CB2R阻断+电针组腹腔注射CB2R阻断剂后行电针干预,5次为1个疗程,共2个疗程。每个疗程结束后间歇1 d进行痛性行为学评价,免疫荧光检测大鼠左侧脊髓背角CB2R表达及脊髓背角神经元CB2R、p-P38和p-ERK定位表达。结果与空白组比较,模型组大鼠左侧机械痛缩足阈和左后肢负重能力显著降低(P<0.01),左侧脊髓背角CB2R表达及CB2R/NeuN、p-P38/NeuN、p-ERK/NeuN双染阳性细胞数显著增加(P<0.05,P<0.01);与模型组比较,电针组、CB2R阻断+电针组大鼠左侧机械痛缩足阈和左后肢负重能力显著升高(P<0.01),且电针组优于CB2R阻断+电针组(P<0.05,P<0.01),电针组大鼠左侧脊髓背角CB2R表达和CB2R/NeuN双染阳性细胞数显著增加(P<0.01),p-P38/NeuN和p-ERK/NeuN双染阳性细胞数显著减少(P<0.01)。结论电针“阳陵泉”“内膝眼”可抑制KOA大鼠痛性行为,其机制可能与激活脊髓背角CB2R,抑制P38/ERK信号通路激活有关。Objective To observe the effects of electroacupuncture on pain behavior,the expressions of cannabinoid receptor 2(CB2R),p-P38 and p-ERK in spinal dorsal horn of rats with knee osteoarthritis(KOA);To explore the mechanism of electroacupuncture in the treatment of KOA chronic pain.Methods Totally 48 SD rats were randomly divided into blank group,saline group,model group,electroacupuncture group,CB2R-blocking group and CB2R-blocking+electroacupuncture group,with 8 rats in each group.Except for the blank group and saline group,monosodium iodoacetate was injected into the left knee joint cavity of the latter four groups to establish the chronic pain model of KOA.The saline was injected into the left knee joint cavity of saline group.15 days after injection,the rats in electroacupuncture group were given electroacupuncture stimulation at left“Yanglingquan”(GB34)and“Neixiyan”(EX-LE4)for 15 minutes,once daily;Cb2R blocker was injected intraperitoneally in CB2R blocking group;CB2R blocking+electroacupuncture group received electroacupuncture intervention after intraperitoneal injection of CB2R blocker.Five times were a course of treatment,with a total of two courses of treatment.The pain behavior was evaluated at an interval of 1 day after each course of treatment.The expressions of CB2R in left dorsal horn of spinal cord and CB2R,p-P38,p-ERK in neurons of spinal dorsal horn were detected by immunofluorescence.Results Compared with the blank group,the mechanical withdrawl threshold of left side and the load-bearing capacity of left hind limbs significantly decreased in the model group(P<0.01),the expression of CB2R,and the number of double staining positive cells of CB2R/NeuN,p-P38/NeuN and p-ERK/NeuN in left dorsal horn of spinal cord increased significantly(P<0.05,P<0.01).Compared with the model group,the mechanical withdrawl threshold of left side and the load-bearing capacity of left hind limbs significantly increased in the electroacupuncture group and CB2R-blocking+electroacupuncture group(P<0.01),and the el

关 键 词：膝骨性关节炎 电针 疼痛 大麻素受体2 P-P38 P-ERK 大鼠 

分 类 号：R245[医药卫生—针灸推拿学] R274.94[医药卫生—中医临床基础]