柴胡疏肝散精简方对慢性疼痛抑郁共病大鼠中缝核P2X7受体、Caspase-1及IL-1β表达的影响  

Effects of Simplified Chaihu Shugan Powder on Expression of P2X7 Receptor,Caspase-1 and IL-1βin Raphe Nucleus of Rats with Pain Accompanied by Depression

作　　者：许丽娥 高雪松[1] 王安娜 杜仪[1] 王永志[1] 赵静洁[1,2] XU Li'e;GAO Xuesong;WANG Anna;DU Yi;WANG Yongzhi;ZHAO Jingjie(Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China;Department of Integrated Traditional Chinese and Western Medicine,Capital Medical University,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京友谊医院,北京100050 [2]首都医科大学中西医结合学系,北京100050

出　　处：《中国中医药信息杂志》2022年第7期99-104,共6页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(82004109);北京市自然科学基金(7204250);首都医科大学临床专科院系开放课题(2020年)。

摘　　要：目的观察柴胡疏肝散精简方对慢性疼痛抑郁共病大鼠行为学及中缝核P2X7受体、半胱氨酸天冬氨酸蛋白酶(Caspase)-1、白细胞介素(IL)-1β表达的影响,探讨其抗疼痛抑郁共病的机制。方法将40只SPF级雄性SD大鼠随机分为空白组、模型组、氟西汀组和柴胡疏肝散精简方组,每组10只。除空白组外,其余3组予利血平腹腔注射造模,氟西汀组和柴胡疏肝散精简方组分别予氟西汀和柴胡疏肝散精简方灌胃,空白组和模型组予等量蒸馏水灌胃,连续14 d。测量大鼠体质量、强迫游泳不动时间、旷场实验运动行为和热缩足阈值;ELISA检测血清IL-6、肿瘤坏死因子(TNF)-α含量;PCR检测中缝核P2X7受体、Caspase-1及IL-1βmRNA表达;Westernblot检测中缝核IL-6、TNF-α蛋白表达。结果与空白组比较,模型组大鼠体质量增量明显减少,强迫游泳不动时间明显增加,旷场实验运动总距离和穿格次数明显减少,热缩足阈值明显降低(P<0.05);血清IL-6、TNF-α含量明显增加(P<0.05),中缝核P2X7受体、Caspase-1及IL-1βmRNA表达明显升高(P<0.05),IL-6、TNF-α蛋白表达明显升高(P<0.05)。与模型组比较,氟西汀组和柴胡疏肝散精简方组大鼠体质量增量明显增加,强迫游泳不动时间明显减少,旷场实验运动总距离和穿格次数明显增加,热缩足阈值明显升高(P<0.05);血清IL-6、TNF-α含量明显减少(P<0.05),中缝核P2X7受体、Caspase-1及IL-1βmRNA表达明显降低(P<0.05),IL-6、TNF-α蛋白表达明显降低(P<0.05)。结论柴胡疏肝散精简方可改善慢性疼痛抑郁共病大鼠行为学及疼痛阈值,其作用机制可能与抑制中缝核P2X7受体表达,进而下调Caspase-1、IL-1β、IL-6和TNF-α表达,减轻炎症反应有关。Objective To observe the effects and mechanism of simplified Chaihu Shugan Powder(SCSP)on behavior and the expression of P2X7 receptor,Caspase-1 and IL-1βin raphe nucleus of rats with pain accompanied by depression;To explore the mechanism of its anti-pain and depression comorbidity.Methods A total of 40 SPF male SD rats were randomly divided into blank group,model group,fluoxetine group and SCSP group,with 10 rats in each group.Except for the blank group,the other 3 groups were given intraperitoneal injection of reserpine for modeling.The fluoxetine group and the SCSP group were given fluoxetine and SCSP Decoction by intragastric administration,respectively.The blank group and model group were given the same volume of distilled water by gavage for 14 consecutive days.Body weight,immobility time of forced swimming,open field motor behavior and thermal contraction threshold in rats were measured.The contents of serum IL-6 and TNFαwere detected by ELISA.The expressions of P2X7 receptor,Caspase-1 and IL-1βmRNA in raphe nucleus were detected by PCR;The expressions of IL-6 and TNFαprotein were detected by Western blot.Results Compared with the blank group,the weight gain of rats in model group decreased significantly(P<0.05),the immobility time of forced swimming significantly increased,the total distance and the number of crossings in the open field test significantly reduced,and the thermal contraction threshold significantly decreased(P<0.05).The contents of serum IL-6 and TNFαsignificantly increased(P<0.05).The expressions of P2X7 receptor,Caspase-1 and IL-1βmRNA in raphe nucleus significantly increased(P<0.05),the expressions of IL-6 and TNFαprotein significantly increased(P<0.05).Compared with the model group,the body weight gain of the rats in fluoxetine group and SCSP group increased significantly,the immobility time of forced swimming significantly reduced,the total distance and the number of crossings of the open field experiment increased significantly,and the thermal contraction threshold increased

关 键 词：柴胡疏肝散精简方 疼痛抑郁共病 P2X7受体 半胱氨酸天冬氨酸蛋白酶-1 白细胞介素-1Β 白细胞介素-6 大鼠 

分 类 号：R285.5[医药卫生—中药学]