Optimising the oil phases of aluminium hydrogel-stabilised emulsions for stable,safe and efficient vaccine adjuvant  

作　　者：Lili Yuan Xiao-Dong Gao Yufei Xia 

机构地区：[1]Key Laboratory of Carbohydrate Chemistry and Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi 214122,China [2]State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China [3]University of Chinese Academy of Sciences,Beijing 100049,China [4]Innovation Academy for Green Manufacture,Chinese Academy of Sciences,Beijing 100190,China

出　　处：《Frontiers of Chemical Science and Engineering》2022年第6期973-984,共12页化学科学与工程前沿（英文版）

基　　金：the Project supported by Beijing Nova Program of Beijing Municipal Science&Technology Commission(Grant No.Z201100006820139);the CAS Project for Young Scientists in Basic Research(YSBR-010);the Pilot Project of Chinese Academy of Sciences(Grant No.XDB29040303);the Foundation for Innovative Research Groups of the National Natural Science Foundation of China(Grant No.21821005);“From 0 to 1”Original Innovation Project of Basic Frontier Scientific Research Program of Chinese Academy of Sciences(Grant No.2020000071);Youth Project of National Natural Science Foundation of China(Grant No.21908229);Youth Innovation Promotion Association of the Chinese Academy of Sciences(Grant No.2020000053).

摘　　要：To increase antibody secretion and dose sparing,squalene-in-water aluminium hydrogel(alun^-stabilised emulsions(ASEs)have been developed,which offer increased surface areas and cellular interactions for higher antigen loading and enhanced immune responses.Nevertheless,the squalene(oil)in previous attempts suffered from limited oxidation resistance,thus,safety and stability were compromised.From a clinical translational perspective,it is imperative to screen the optimal oils for enhanced emulsion adjuvants.Here,because of the varying oleic to linoleic acid ratio,soybean oil,peanut oil,and olive oil were utilised as oil phases in the preparation of aluminium hydrogel-stabilised squalene-in-water emulsions,which were then screened for their stability and immunogenicity.Additionally,the underlying mechanisms of oil phases and emulsion stability were unravelled,which showed that a higher oleic to linoleic acid ratio increased anti-oxidative capabilities but reduced the long-term storage stability owing to the relatively low zeta potential of the prepared droplets.As a result,compared with squalene-in-water ASEs,soybean-in-water ASEs exhibited comparable immune responses and enhanced stability.By optimising the oil phase of the emulsion adjuvants,this work may offer an alternative strategy for safe,stable,and effective emulsion adjuvants.

关 键 词：pickering emulsion vaccine adjuvant alum-stabilised emulsion oleic to linoleic acid ratio stability 

分 类 号：TQ427.26[化学工程]